Volume 19 Issue 6
Jun.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(6): 990-993
WU Xiao-yu, CUI Fa-cai, HU Min, ZHU Ya, ZHENG Pei-ming. Expression and clinical diagnostic of serum miR-92a-1 in patients with colorectal cancer[J]. Chinese Journal of General Practice, 2021, 19(6): 990-993. doi: 10.16766/j.cnki.issn.1674-4152.001968
Citation: WU Xiao-yu, CUI Fa-cai, HU Min, ZHU Ya, ZHENG Pei-ming. Expression and clinical diagnostic of serum miR-92a-1 in patients with colorectal cancer[J]. Chinese Journal of General Practice, 2021, 19(6): 990-993. doi: 10.16766/j.cnki.issn.1674-4152.001968
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Expression and clinical diagnostic of serum miR-92a-1 in patients with colorectal cancer

doi: 10.16766/j.cnki.issn.1674-4152.001968

  • Department of Clinical Laboratory, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, China

Funds:

 81802094

  • Received Date: 2020-10-11
    Available Online: 2022-02-16
    Abstract
  •   Objective  This study aimed to investigate the expression of serum miR-92a-1 and its clinical diagnostic value in patients with colorectal cancer (CRC).   Methods  The serum samples of 131 patients with CRC and 50 patients with colorectal adenomas (CRA), who were hospitalised from May 2018 to December 2019 at the Henan Provincial People's Hospital, were collected, and 50 healthy controls were selected. The expression of serum miR-92a-1, carcinoma embryonic antigen (CEA) and carbohydrate antigen 199 (CA199) were detected by real-time quantitative PCR and electrochemiluminescence (ECLIA) and compared amongst the three groups. The relationship between serum miR-92a-1 and the clinicopathological characteristics of CRC patients was further analysed, and the receiver operating curves (ROC) were constructed to assess the diagnostic value of miR-92a-1 or combination of CEA and CA199 in CRC.   Results  The expression level of serum miR-92a-1, CEA and CA-199 in CRC patients was significantly higher than those in CRA patients or healthy controls, with statistically significant differences (all P < 0.05). The expression of serum miR-92a-1 in CRC patients was correlated with the degree of differentiation, TNM stage, lymph node metastasis and distant metastasis (all P < 0.05) but not with gender, age, tumour diameter and high risk (all P>0.05). The area under ROC curve (AUC) of miR-92a-1 in the diagnosis of CRC was 0.875 (95% CI: 0.833-0.918), and the sensitivity and specificity were 72.5% and 86%, respectively. The AUC of miR-92a-1 combined with CEA and CA-199 in the diagnosis of CRC was 0.904 (95% CI: 0.865-0.943), and sensitivity and specificity were increased to 80.2% and 91%, respectively.   Conclusion  The high expression of miR-92a-1 in CRC is related to the degree of differentiation, TNM stage, lymph node metastasis and distant metastasis. miR-92a-1 combined with conventional tumour marker detection has a high diagnostic value for CRC.

     

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