Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma

WANG Hanlin; HONG Yuan; TANG Jiaxin; ZHANG Renquan

doi:10.16766/j.cnki.issn.1674-4152.004439

Volume 24 Issue 4

Apr. 2026

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Article Navigation > Chinese Journal of General Practice > 2026 > 24(4): 559-563

WANG Hanlin, HONG Yuan, TANG Jiaxin, ZHANG Renquan. Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma[J]. Chinese Journal of General Practice, 2026, 24(4): 559-563. doi: 10.16766/j.cnki.issn.1674-4152.004439

Citation:

WANG Hanlin, HONG Yuan, TANG Jiaxin, ZHANG Renquan. Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma[J]. Chinese Journal of General Practice, 2026, 24(4): 559-563. doi: 10.16766/j.cnki.issn.1674-4152.004439

Citation:

WANG Hanlin, HONG Yuan, TANG Jiaxin, ZHANG Renquan. Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma[J]. Chinese Journal of General Practice, 2026, 24(4): 559-563. doi: 10.16766/j.cnki.issn.1674-4152.004439

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Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma

doi: 10.16766/j.cnki.issn.1674-4152.004439

1.
Department of General Thoracic Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230002, China

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202304295107020047

S202410366057

Received Date: 2025-05-06

Abstract

Abstract

Objective To analyze the expression of transmembrane protein 63C (TMEM63C) in lung adenocarcinoma (LUAD), its relationship with patient prognosis, and its effects on the proliferation, migration, and invasion of LUAD cells, as well as the underlying molecular mechanisms. Methods TMEM63C expression in LUAD cells was detected by RT qPCR and Western Blotting. Its expression and prognostic value in LUAD tissues were analyzed using the GEPIA, MetaIntegrator, Kaplan Meier plotter databases, and Cox regression. Immune infiltration and enrichment analyses were performed with the TIMER, LinkedOmics, and Metascape databases. Following siRNA interference, CCK-8, colony formation, wound healing, Transwell, and Western Blotting assays were used to assess changes in cell proliferation, migration, invasion, and PI3K/AKT protein expression. Results TMEM63C was highly expressed in LUAD, and elevated expression was significantly associated with improved overall survival (P < 0.05). Multivariate Cox regression analysis showed that high expression of TMEM63C was an independent protective factor for overall survival in LUAD patients [HR (95% CI): 0.798 (0.686~0.928), P=0.003]. TMEM63C expression was positively correlated with infiltration of NK cells (r=0.275) and dendritic cells (r=0.188), and negatively correlated with neutrophil infiltration (r=-0.106, P < 0.05). Knockdown of TMEM63C significantly promoted LUAD cell proliferation, migration, and invasion, accompanied by increased expression of PI3K and AKT proteins (P < 0.05). Conclusion TMEM63C is highly expressed in LUAD and is associated with a favorable patient prognosis. Its tumor-suppressive effects may be mediated through modulating of the tumor microenvironment and inhibiting of the PI3K/AKT signaling pathway.
- Lung adenocarcinoma,
- Transmembrane protein 63C,
- Tumour microenvironment,
- PI3K/AKT signaling,
- Immune infiltration,
- Prognosis

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Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma

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Study on the expression characteristics, prognostic value, and potential mechanisms of TMEM63C in lung adenocarcinoma

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