Volume 24 Issue 3
Mar.  2026
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2026  >  24(3): 395-398
CAI Fengjiao, WU Jinhong, WANG Lili, MA Yichao, LU Yan. The role of TRIM62 in regulating autophagy in aortic smooth muscle cells through the mTOR pathway[J]. Chinese Journal of General Practice, 2026, 24(3): 395-398. doi: 10.16766/j.cnki.issn.1674-4152.004404
Citation: CAI Fengjiao, WU Jinhong, WANG Lili, MA Yichao, LU Yan. The role of TRIM62 in regulating autophagy in aortic smooth muscle cells through the mTOR pathway[J]. Chinese Journal of General Practice, 2026, 24(3): 395-398. doi: 10.16766/j.cnki.issn.1674-4152.004404
shu

The role of TRIM62 in regulating autophagy in aortic smooth muscle cells through the mTOR pathway

doi: 10.16766/j.cnki.issn.1674-4152.004404
  • 1.

    First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China

Funds:

 202103021224393

  • Received Date: 2025-09-15
    Available Online: 2026-06-02
    Abstract
  •   Objective  To explore the role of TRIM62 in regulating autophagy in aortic smooth muscle cells (ASMCs) and its potential mechanisms, and to evaluate its impact on vascular calcification.  Methods  Aortic smooth muscle cells were isolated and cultured from Sprague-Dawley rats. Stable cell lines were constructed for overexpression of TRIM62 (OE-TRIM62), empty vector control (Vector), knockout of TRIM62 (KO-TRIM62), and negative control (NC) groups with 12 samples in each group. Vascular calcification was induced using sodium β-glycerophosphate. Autophagy levels were assessed by monodansylcadaverine (MDC) fluorescent flow cytometry, observation of autophagosomes by transmission electron microscopy, LC3 fluorescent imaging, and adenosine triphosphate (ATP) activity assay. The expression of autophagy-related proteins (P62, ATG3, LC3) and mammalian target of rapamycin(mTOR) signaling pathway proteins (mTOR, p-mTOR) was detected by Western blot (WB).  Results  Compared with the Vector group, the median fluorescence intensity of MDC in OE-TRIM62 group was significantly increased (156.7±12.4 vs. 108.6±9.1, P < 0.05), and transmission electron microscopy showed that the number of autophagosomes increased. The ratio of p-mTOR/mTOR significantly increased (1.12±0.09 vs. 0.86±0.07, P < 0.05), and the relative expression levels of P62, ATG3, and LC3-Ⅱ proteins significantly decreased (P < 0.05). The rate of LC3 positive cells was significantly increased (24.3%±2.7% vs. 13.1%±1.8%, P < 0.05), and ATP activity was significantly decreased (P < 0.05). The p-mTOR/mTOR ratio was significantly decreased (P < 0.05), and the relative expressions of P62, ATG3 and LC3-Ⅱ proteins were significantly increased (P < 0.05). Compared with NC group, the median fluorescence intensity of MDC in KO-TRIM62 group was significantly decreased (78.4±6.2 vs. 105.3±8.7, P < 0.05). Transmission electron microscopy showed that the number of autophagosomes and the rate of LC3 positive cells were significantly decreased (6.8%±0.9% vs. 12.5%±1.6%, P < 0.05), the p-mTOR/mTOR ratio was significantly increased (P < 0.05), and the relative expressions of P62, ATG3 and LC3-Ⅱ proteins were significantly decreased (P < 0.05).  Conclusion  TRIM62 may promote the autophagy process in ASMCs by inhibiting the p-mTOR-dependent autophagy pathway, thereby relieving the suppression of autophagy by the mTOR pathway. This, in turn, may be involved in regulating the process of vascular calcification.

     

    • FullText(HTML)
  • loading
    • References(21)
  • [1]
    VILLA-BELLOSTA R. Vascular calcification: key roles of phosphate and pyrophosphate[J]. Int J of Mol Sci, 2021, 22(24): 13536. DOI: 10.3390/ijms222413536.
    [2]
    LI M, WANG Z W, FANG L J, et al. Programmed cell death in atherosclerosis and vascular calcification[J]. Cell Death Dis, 2022, 13(5): 467. DOI: 10.1038/s41419-022-04923-5.
    [3]
    YIN Z, ZHANG J S, SHEN Z C, et al. Regulated vascular smooth muscle cell death in vascular diseases[J]. Cell Prolif, 2024, 57(11): e13688. DOI: 10.1111/cpr.13688.
    [4]
    LIU N, YANG Y, LI X, et al. PARylation of POLG mediated by PARP1 accelerates ferroptosis-induced vascular calcification via activating Adora2a/Rap1 signaling[J]. Arterioscler Thromb Vasc Biol, 2025, 45(6): 1207-1221.
    [5]
    ZHANG L, LI D Z, AIERKEN Y, et al. KPV and RAPA self-assembled into carrier-free nanodrugs for vascular calcification therapy[J]. Adv Healthc Mater, 2024, 13(32): e2402320. DOI: 10.1002/adhm.202402320.
    [6]
    王琳茹, 张晶, 赵冬婵, 等. 沉默FOXO1基因对人主动脉血管平滑肌细胞自噬和凋亡的影响[J]. 吉林大学学报(医学版), 2024, 50(2): 431-441.

    WANG L R, ZHANG J, ZHAO D C, et al. Effect of silencing FOXO1 gene on autophagy and apoptosis in human aortic vascular smooth muscle cells[J]. Journal of Jilin University (Medicine Edition), 2024, 50(2): 431-441.
    [7]
    LI P, DONG X R, ZHANG B, et al. Molecular mechanism and therapeutic targeting of necrosis, apoptosis, pyroptosis, and autophagy in cardiovascular disease[J]. Chin Med J (Engl), 2021, 134(22): 2647-2655. doi: 10.1097/CM9.0000000000001772
    [8]
    KLIONSKY D J, PETRONI G, AMARAVADI R K, et al. Autophagy in major human diseases[J]. EMBO J, 2021, 40(19): e108863. DOI: 10.15252/embj.2021108863.
    [9]
    LI X, WANG X, LIU Y, et al. Downregulation of miR-3568 protects against ischemia/reperfusion-induced cardiac dysfunction in rats and apoptosis in H9C2 cardiomyocytes through targeting TRIM62[J]. Front Pharmacol, 2020, 11: 17. DOI: 10.3389/fphar.2020.00017.
    [10]
    ONNIS C, VIRMANI R, KAWAI K, et al. Coronary artery calcification: current concepts and clinical implications[J]. Circulation, 2024, 149(3): 251-266. doi: 10.1161/CIRCULATIONAHA.123.065657
    [11]
    MOSQUREA J V, AUGUSTE G, WONG D, et al. Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis[J]. Cell Rep, 2023, 42(11): 113380. DOI: 10.1016/j.celrep.2023.113380.
    [12]
    王艺凯, 郭利伟. 成纤维细胞因子23-Klotho轴在血管钙化中的作用机制研究进展[J]. 新乡医学院学报, 2023, 40(11): 1083-1086.

    WANG Y K, GUO L W. Research progress on the mechanism of fibroblast growth factor 23-Klotho axis in vascular calcification[J]. Journal of Xinxiang Medical University, 2023, 40(11): 1083-1086.
    [13]
    VARGAS J N S, HAMASAKI M, KAWABATA T, et al. The mechanisms and roles of selective autophagy in mammals[J]. Nat Rev Mol Cell Biol, 2023, 24(3): 167-185.
    [14]
    AHSAN N, SHARIQ M, SUROLIA A, et al. Multipronged regulation of autophagy and apoptosis: emerging role of TRIM proteins[J]. Cell Mol Biol Let, 2024, 29(1): 13. DOI: 10.1186/s11658-023-00528-8.
    [15]
    TANG J, CHEN Q, XIANG L, et al. TRIM28 Fosters microglia ferroptosis via autophagy modulation to enhance neuropathic pain and neuroinflammation[J]. Mol Neurobiol, 2024, 61(11): 9459-9477. doi: 10.1007/s12035-024-04133-4
    [16]
    CHEN J, CAO W, HUANG X, et al. TRIM21 enhances bortezomib sensitivity in multiple myeloma by halting prosurvival autophagy[J]. Blood Adv, 2023, 7(19): 5752-5770. doi: 10.1182/bloodadvances.2022008241
    [17]
    张永琪, 邹震海, 吴梦琦, 等. 敲低EPHA2通过mTOR磷酸化调控膀胱癌细胞自噬和生物学行为[J]. 中华全科医学, 2023, 21(7): 1117-1120, 1129. doi: 10.16766/j.cnki.issn.1674-4152.003063

    ZHANG Y Q, ZOU Z H, WU M Q, et al. Knockdown of EPHA2 regulates autophagy and biological behavior of bladder cancer cells through mTOR phosphorylation[J]. Chinese Journal of General Practice, 2023, 21(7): 1117-1120, 1129. doi: 10.16766/j.cnki.issn.1674-4152.003063
    [18]
    XU Y F, WANG Q, WANG J, et al. The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance[J]. Immunity, 2025, 58(2): 309-325.
    [19]
    ZHOU X, XU S N, YUAN S T, et al. Multiple functions of autophagy in vascular calcification[J]. Cell Biosci, 2021, 11(1): 159. DOI: 10.1186/s13578-021-00639-9.
    [20]
    LI F X Z, LIU J J, XU F, et al. Cold exposure protects against medial arterial calcification development via autophagy[J]. J Nanobiotechnol, 2023, 21(1): 226. DOI: 10.1186/s12951-023-01985-1.
    [21]
    ZHOU Z K, LI Y, JIANG W, et al. Molecular mechanism of calycosin inhibited vascular calcification[J]. Nutrients, 2023, 16(1): 99. DOI: 10.3390/nu16010099.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(2)  / Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (2) PDF downloads(0) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return