Study on the relationship between the nature of cold and heat of IBS-D and changes in gut microbiota and metabolites

TANG Binbin; HU Yunlian; SU Chengxia; HUANG Chaoqun; WEN Na

doi:10.16766/j.cnki.issn.1674-4152.004199

Volume 29 Issue 10

Oct. 2025

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TANG Binbin, HU Yunlian, SU Chengxia, HUANG Chaoqun, WEN Na. Study on the relationship between the nature of cold and heat of IBS-D and changes in gut microbiota and metabolites[J]. Chinese Journal of General Practice, 2025, 23(10): 1658-1662. doi: 10.16766/j.cnki.issn.1674-4152.004199

Citation:

TANG Binbin, HU Yunlian, SU Chengxia, HUANG Chaoqun, WEN Na. Study on the relationship between the nature of cold and heat of IBS-D and changes in gut microbiota and metabolites[J]. Chinese Journal of General Practice, 2025, 23(10): 1658-1662. doi: 10.16766/j.cnki.issn.1674-4152.004199

Citation:

TANG Binbin, HU Yunlian, SU Chengxia, HUANG Chaoqun, WEN Na. Study on the relationship between the nature of cold and heat of IBS-D and changes in gut microbiota and metabolites[J]. Chinese Journal of General Practice, 2025, 23(10): 1658-1662. doi: 10.16766/j.cnki.issn.1674-4152.004199

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Study on the relationship between the nature of cold and heat of IBS-D and changes in gut microbiota and metabolites

doi: 10.16766/j.cnki.issn.1674-4152.004199

TANG Binbin¹
, HU Yunlian^{2, 3, 4}
, SU Chengxia^{2, 3}
, HUANG Chaoqun^{2, 3}
, WEN Na^{2, 3
,
,}

1.
Second Outpatient Department, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, China

Funds:

82374205

2023ZR004

2024KY869

2022020801020508

Received Date: 2024-10-10

Abstract

Abstract

Objective To investigate the correlation between changes in gut microbiota and metabolites and the nature of cold and heat in traditional Chinese medicine (TCM) through diarrhea-predominant irritable bowel syndrome (IBS-D) rat models with cold-dampness syndrome and damp-heat syndrome. Methods Thirty newborn rats were divided into a healthy group, a damp-heat group, and a cold-dampness group using the random number table method, with 10 rats in each group. The damp-heat group was subjected to maternal separation, restraint stress, a high-fat and high-sugar diet, and a damp-heat environment to establish an IBS-D rat model with damp-heat syndrome. The cold-dampness group was subjected to maternal separation, restraint stress, and intragastric administration of senna leaves to establish an IBS-D rat model with cold-dampness syndrome. Subsequently, 16S rRNA sequencing and non-targeted metabolomics were employed to detect the gut microbiota and fecal metabolites of the rats in each group, respectively, followed by bioinformatics analysis. Results Significant differences in β-diversity were observed among the rat groups (P=0.001). Specifically, bacterial genera such as Clostridium_sensu_stricto_1, Roseburia, and Prevotella were enriched in the damp-heat group, whereas Dubosiella, Akkermansia, and Romboutsia were enriched in the cold-dampness group. The disrupted metabolic pathways in the damp-heat group rats primarily involved alanine, aspartate, and glutamate metabolism. In contrast, the cold-dampness group rats exhibited disruptions in steroid hormone biosynthesis, tryptophan metabolism, and histidine metabolism. The differential pathways between the damp-heat and cold-dampness groups mainly involved pantothenate and CoA biosynthesis, histidine metabolism, and glycerophospholipid metabolism. Conclusion There are differences in the characteristics of gut microbiota and metabolites between IBS-D with cold-dampness syndrome and damp-heat syndrome. The changes in gut microbiota and metabolites might be the microscopic manifestations of the nature of cold and heat in TCM.
- Irritable bowel syndrome,
- Gut microbiota,
- Metabolomics,
- Traditional Chinese medicine,
- Nature of cold and heat in disease

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References(23)

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