Volume 23 Issue 5
May  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(5): 760-764
ZHANG Liao, FU Keshang, DENG Yingping, JIN Yong, WU Quan, SHI Yanhong. The effect of Bushen Zhuanggu Decoction on the extracellular matrix homeostasis of chondrocytes in osteoarthritis rats[J]. Chinese Journal of General Practice, 2025, 23(5): 760-764. doi: 10.16766/j.cnki.issn.1674-4152.003995
Citation: ZHANG Liao, FU Keshang, DENG Yingping, JIN Yong, WU Quan, SHI Yanhong. The effect of Bushen Zhuanggu Decoction on the extracellular matrix homeostasis of chondrocytes in osteoarthritis rats[J]. Chinese Journal of General Practice, 2025, 23(5): 760-764. doi: 10.16766/j.cnki.issn.1674-4152.003995
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The effect of Bushen Zhuanggu Decoction on the extracellular matrix homeostasis of chondrocytes in osteoarthritis rats

doi: 10.16766/j.cnki.issn.1674-4152.003995
  • 1.

    Department of Orthopaedics, Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Ningbo, Zhejiang 315000, China

Funds:

 GZY-ZJ-KJ-23037

 2024ZL144

 2021J300

  • Received Date: 2024-05-15
    Available Online: 2025-08-14
    Abstract
  •   Objective  To investigate the effect and mechanism of Bushen Zhuanggu Decoction on the imbalance of extracellular matrix homeostasis in chondrocytes of osteoarthritis (OA) rats.  Methods  Thirty-six rats were randomly divided into six groups using a random number table: sham group, model group, Bushen Zhuanggu Decoction low-dose, medium-dose, high-dose group, and positive control group, with six in each group. Histological changes in cartilage tissue were evaluated using HE and Safranin O staining. The expression of proteins and mRNA related to the Wnt/β-catenin signaling pathway and cartilage degradation was assessed using Western blotting and RT-PCR, respectively.  Results  The model group exhibited significant cartilage surface fibrosis and reduced chondrocyte numbers. In contrast, the low-, medium-, and high-dose Bushen Zhuanggu Decoction groups, as well as the positive control group, showed an orderly arrangement of chondrocytes, with the most pronounced pathological improvement observed in the high-dose group. Compared to the model group (12.49±1.34), the Mankin ' s scores significantly decreased in the low-dose (10.17±1.19), medium-dose (7.50±0.89), high-dose (3.99±0.45), and positive control groups (6.51±0.73). The expression levels of IL-1β, Wnt5α, β-catenin, GSK-3β, MMP-13, and ADAMTS5 proteins and mRNA in cartilage tissue were significantly reduced, while the expression of Axin, Collagen Ⅱ, and Aggrecan proteins and mRNA were significantly increased (P < 0.05).  Conclusion  Bushen Zhuanggu Decoction inhibits the degradation of the extracellular matrix in chondrocytes of OA rats and exerts therapeutic effects. Its mechanism may be related to the inhibition of the Wnt/β-catenin signaling pathway.

     

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