Volume 21 Issue 6
Jun.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(6): 1060-1063
LI Jun, TAO Run, WANG Weijie, ZHANG Jiajun. Analyze the core gene of renal clear cell carcinoma based on bioinformatics and verify the expression of AURKB[J]. Chinese Journal of General Practice, 2023, 21(6): 1060-1063. doi: 10.16766/j.cnki.issn.1674-4152.003049
Citation: LI Jun, TAO Run, WANG Weijie, ZHANG Jiajun. Analyze the core gene of renal clear cell carcinoma based on bioinformatics and verify the expression of AURKB[J]. Chinese Journal of General Practice, 2023, 21(6): 1060-1063. doi: 10.16766/j.cnki.issn.1674-4152.003049
shu

Analyze the core gene of renal clear cell carcinoma based on bioinformatics and verify the expression of AURKB

doi: 10.16766/j.cnki.issn.1674-4152.003049

  • Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2020A1290

  • Received Date: 2022-08-09
    Available Online: 2023-08-26
    Abstract
  •   Objective  The target gene AURKB was screened in renal clear cell carcinoma by bioinformatics method, and its clinical significance in renal clear cell carcinoma was analyzed.  Methods  The GSE66271 dataset was downloaded from GEO database and the differential genes were screened. Hub genes were selected based on STRING database and Cytoscape software. The expression and prognostic relationship of AURKB gene in renal clear cell carcinoma were verified by GEPIA online database. The difference of AURKB expression in clear cell carcinoma and adjacent normal tissue was verified by immunohistochemistry.  Results  A total of 517 differential genes were obtained after analysis of GSE66271 data set, including 155 up-regulated genes and 362 down-regulated genes. AURKB was selected from 10 hub genes in the differential gene network for verification. GEPIA database analysis showed that AURKB high expression significantly reduced the overall survival (OS) and disease-free survival (DFS) in KIRC patients (P < 0.05), indicating that AURKB may be a KIRC oncogenic gene. In addition, AURKB expression was positively correlated with different disease stages in KIRC patients (P < 0.05). Immunohistochemical test showed that AURKB protein was mainly expressed in the nucleus, and the positive expression in cancer tissues (58.00%, 29/50) was higher than that in adjacent normal tissues (24.00%, 12/50), the difference was statistically significant (χ2=11.947, P=0.001).  Conclusion  AURKB is highly expressed in renal clear cell carcinoma and leads to poor prognosis, suggesting that AURKB may be an oncogenic genes for KIRC, and is expected to be a new therapeutic target for KIRC.

     

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