Volume 23 Issue 8
Aug.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(8): 1377-1381
GONG Zhiming, HUANG Feiwu, ZHAO Yunan, CHEN Jiaoli, ZHAO Xinmi, XU Jianfen, HUANG Jiansheng. The molecular epidemiology of carbapenem-resistant Citrobacter freundii in Lishui City[J]. Chinese Journal of General Practice, 2025, 23(8): 1377-1381. doi: 10.16766/j.cnki.issn.1674-4152.004138
Citation: GONG Zhiming, HUANG Feiwu, ZHAO Yunan, CHEN Jiaoli, ZHAO Xinmi, XU Jianfen, HUANG Jiansheng. The molecular epidemiology of carbapenem-resistant Citrobacter freundii in Lishui City[J]. Chinese Journal of General Practice, 2025, 23(8): 1377-1381. doi: 10.16766/j.cnki.issn.1674-4152.004138
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The molecular epidemiology of carbapenem-resistant Citrobacter freundii in Lishui City

doi: 10.16766/j.cnki.issn.1674-4152.004138

  • Department of Clinical Laboratory, Lishui Central Hospital, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang 323000, China

Funds:

 LHDMZ23H190001

 LHDMY24H190001

 2024XY103

 2023ZDYF16

 2023GYX43

 2024SJZC102

  • Received Date: 2024-12-03
    Available Online: 2025-10-31
    Abstract
  •   Objective  To analyze the resistance mechanisms and molecular epidemiological characteristics of carbapenem-resistant Citrobacter freundii (CRCF), providing laboratory evidence for clinical prevention and treatment.  Methods  CRCF strains collected from January 2016 to December 2022 in Lishui Central Hospital were tested for MIC using the broth microdilution method. PCR was employed to detect carbapenemase genes (blaKPC-2, blaNDM-1, blaIMP-1, blaVIM-1, blaOXA-48), and the genetic background of resistance genes. Conjugation and transformation experiments were conducted to analyze plasmid transferability and plasmid typing. MLST typing was combined with CRCF strains from the PubMed database for phylogenetic analysis.  Results  The resistance rates of 17 CRCF isolates were 100% (17/17) to trimethoprim/sulfamethoxazole; 76.5% (13/17) to ceftazidime, cefepime, and aztreonam; 82.4% (14/17) to meropenem; 64.7% (11/17) to imipenem; 94.1% (16/17) to piperacillin/tazobactam; 58.8% (10/17) to LVX; 47.1% (8/17) to amikacin; 11.8% (2/17) to polymyxin; and the lowest resistance rate was to tigecycline at 5.9% (1/17). Among these strains, 11 carried blaNDM-1, 5 carried blaKPC-2, and 1 carried blaIMP-4. 54.5%(6/11)of blaNDM-1 were located on IncX3 plasmids, while all of blaKPC-2 were located on IncFⅡ plasmids. The strains were classified into 14 ST types, with phylogenetic analysis indicating sporadic distribution.  Conclusion  CRCF in Lishui exhibits high resistance to carbapenem antibiotics, primarily carrying blaNDM-1 and blaKPC-2, presenting a sporadic epidemic pattern.

     

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