Volume 23 Issue 1
Jan.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(1): 85-88
JIANG Yazhou, ZHU Suyue, QIAO Jibing, SHEN Nan, WU Rang, LIU Feng. Effects of hand, foot, and mouth disease on oral microbiota in children[J]. Chinese Journal of General Practice, 2025, 23(1): 85-88. doi: 10.16766/j.cnki.issn.1674-4152.003841
Citation: JIANG Yazhou, ZHU Suyue, QIAO Jibing, SHEN Nan, WU Rang, LIU Feng. Effects of hand, foot, and mouth disease on oral microbiota in children[J]. Chinese Journal of General Practice, 2025, 23(1): 85-88. doi: 10.16766/j.cnki.issn.1674-4152.003841
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Effects of hand, foot, and mouth disease on oral microbiota in children

doi: 10.16766/j.cnki.issn.1674-4152.003841
  • 1.

    Department of Respiration, Children′ s Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, China

Funds:

 F201941

 F202153

  • Received Date: 2024-02-24
    Available Online: 2025-02-13
    Abstract
  •   Objective  To detect and analyze the changes in oral microbiota of children with hand, foot, and mouth disease (HFMD) by high-throughput sequencing technology.   Methods   A total of 86 children diagnosed with HFMD in Suqian Hospital Affiliated to Xuzhou Medical University from April 2021 to September 2023, as well as 38 healthy children who underwent physical examinations during the same period, were selected as the HFMD group and the control group, respectively. Bilateral buccal mucosal saliva swabs of all the included children were collected and genomic DNA was extracted. 16S rDNA amplicon sequencing was performed after quality inspection. The biological information obtained by sequencing was clustered, annotated, and compared between groups. α diversity analysis and β diversity analysis were used to compare the abundance, diversity, and structural changes. The top ten abundant key bacteria genera of the two groups were significantly compared again to screen biomarkers.   Results   A total of 1 891 OTUs were obtained in the two groups, 1 047 in the HFMD group, and 844 in the control group, with 544 between the groups. Compared to healthy children, the oral microbiota structure of HFMD was changed, and the shannon index (P < 0.05) and ACE index (P < 0.05) were significantly decreased. The key bacterial genera are characterized by a decrease in the abundance of Leptotrichia (P < 0.05) and Capnocytophaga (P < 0.05).   Conclusion   The oral microbiota of HFMD is disordered, with significantly reduced diversity and richness, characterized by a decrease in short-chain fatty acids-producing bacteria. These results can provide some theoretical basis for the study of HFMD microecology mechanism and its targeted intervention in the future.

     

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