Volume 19 Issue 12
Dec.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(12): 2031-2034, 2097
PAN Han-song, GUO Chang-jun, LIANG Yi-min, LIANG Jian-wei, LI Xiang. Effects of the up-regulation of KLF13 on invasion, migration and p-AKT expression of osteosarcoma cells[J]. Chinese Journal of General Practice, 2021, 19(12): 2031-2034, 2097. doi: 10.16766/j.cnki.issn.1674-4152.002228
Citation: PAN Han-song, GUO Chang-jun, LIANG Yi-min, LIANG Jian-wei, LI Xiang. Effects of the up-regulation of KLF13 on invasion, migration and p-AKT expression of osteosarcoma cells[J]. Chinese Journal of General Practice, 2021, 19(12): 2031-2034, 2097. doi: 10.16766/j.cnki.issn.1674-4152.002228
shu

Effects of the up-regulation of KLF13 on invasion, migration and p-AKT expression of osteosarcoma cells

doi: 10.16766/j.cnki.issn.1674-4152.002228

  • Department of Orthopaedics, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, China

Funds:

 LY20H070006

 1701KY48

  • Received Date: 2020-06-18
    Available Online: 2022-03-02
    Abstract
  •   Objective  To study the effects of Kruppel-like factor 13 (KLF13) on the invasion and migration of osteosarcoma cells (MG-63) and changes in p-AKT protein expression during the process.  Methods  MG-63 cells were divided into control group, NC group and LV-KLF13-OE group according to random number table method. The transfection of KLF13 recombinant lentivirus particles was observed under a microscope. The expression levels of KLF13 mRNA were detected by real-time fluorescent quantitative PCR. In addition, the protein expression levels of KLF13, serine/threonine protein kinase B (AKT) and its phosphorylation, E-cadherin and membrane palmitoylated protein 2 (MPP-2) were detected by western blot. Proliferation was detected by the Cell Counting Kit-8 (CCK-8), the migration ability was detected by a scratch test, and invasiveness was detected by the transwell method.  Results  The transfection efficiency of MG-63 cells was over 75%. Compared with those in the control group and NC group, the expression levels of KLF13 mRNA and protein and E-cadherin protein in the LV-KLF13-OE group were significantly higher (F=1 544.143, 588.000, 235.391, all P < 0.001), and the expression levels of MPP-2 protein were significantly lower (F=260.053, P < 0.001). The scratch healing rate [(20.07±8.18) %], number of invasions (89.29±15.04) and p-AKT protein expression (0.21±0.02) levels in the LV-KLF13-OE group were significantly lower than those in the control group [(71.61±6.02)%, 221.33±20.09 and 1.12±0.12] and NC group [(74.93±7.21)%, 212.69±25.47 and 1.09±0.08; F=109.781, 76.815, 226.896, all P < 0.001].  Conclusion  KLF13 overexpression may inhibit the migration and invasion of human osteosarcoma cells by down-regulating p-AKT expression.

     

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