Volume 19 Issue 12
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QI Yu-jun, LI Jie, LI Lin, CHEN Meng, LIU Jian-ping. MTP18 promotes oral squamous-cell carcinoma metastasis through induction of epithelial-mesenchymal transition[J]. Chinese Journal of General Practice, 2021, 19(12): 2003-2007. doi: 10.16766/j.cnki.issn.1674-4152.002221
Citation: QI Yu-jun, LI Jie, LI Lin, CHEN Meng, LIU Jian-ping. MTP18 promotes oral squamous-cell carcinoma metastasis through induction of epithelial-mesenchymal transition[J]. Chinese Journal of General Practice, 2021, 19(12): 2003-2007. doi: 10.16766/j.cnki.issn.1674-4152.002221
shu

MTP18 promotes oral squamous-cell carcinoma metastasis through induction of epithelial-mesenchymal transition

doi: 10.16766/j.cnki.issn.1674-4152.002221
  • 1.

    Department of Stomatology, Northwestern Polytechnical University Hospital, Xi'an, Shaanxi 710072, China

Funds:

 2018JM7018

  • Received Date: 2020-04-27
    Available Online: 2022-03-02
    Abstract
  •   Objective  To explore the biological functions of mitochondrial protein 18 (MTP18) in oral squamous-cell carcinoma metastasis.  Methods  (1) Bioinformatic analysis was conducted to evaluate the expression of MTP18 in 44 normal oral mucosa and 520 oral squamous-cell carcinoma tissues. (2) The silencing effect of MTP18 by siRNA was measured by qRT-PCR and Western blot analysis. In addition, the effects of MTP18 knockdown on the migration and invasion abilities of oral squamous-cell carcinoma were determined by wound-healing and Transwell invasion assays. (3) The effects of MTP18 knockdown on the expressions of markers in epithelial-mesenchymal transition (EMT) were determined by qRT-PCR analysis.  Results  (1) Bioinformatic analysis indicated that MTP18 expression was significantly increased in tumour tissues of oral squamous-cell carcinoma, especially in tumour tissues at high stages and with lymph node metastasis, compared with normal tissues (P < 0.001). (2) siRNA transfection successfully reduced the expression of MTP18 in oral squamous-cell carcinoma cells (P < 0.001). MTP18 knockdown significantly suppressed cell migration (siCtrl vs. siMTP18#1 vs. siMTP18#2: 1.00±0.07 vs. 0.43±0.04 vs. 0.44±0.03, F=102.241, P < 0.001) and invasion (siCtrl vs. siMTP18#1 vs. siMTP18#2: 1.00±0.07 vs. 0.43±0.04 vs. 0.44±0.03, F=97.017, P < 0.001). (3) MTP18 knockdown significantly increased the expressions of epithelial cell markers (P < 0.001), but suppressed the expressions of mesenchymal cell markers (P < 0.001).  Conclusion  MTP18 overexpression promotes oral squamous-cell carcinoma metastasis mainly through the induction of EMT.

     

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