MiR-182 enhances and promotes the proliferation and invasion of melanoma cells

LI Xu-wen; SONG Pei-jun; LI Wei; XU Jing; ZHANG Li

doi:10.16766/j.cnki.issn.1674-4152.002089

Volume 19 Issue 9

Sep. 2021

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Article Navigation > Chinese Journal of General Practice > 2021 > 19(9): 1474-1476,1548

LI Xu-wen, SONG Pei-jun, LI Wei, XU Jing, ZHANG Li. MiR-182 enhances and promotes the proliferation and invasion of melanoma cells[J]. Chinese Journal of General Practice, 2021, 19(9): 1474-1476,1548. doi: 10.16766/j.cnki.issn.1674-4152.002089

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LI Xu-wen, SONG Pei-jun, LI Wei, XU Jing, ZHANG Li. MiR-182 enhances and promotes the proliferation and invasion of melanoma cells[J]. Chinese Journal of General Practice, 2021, 19(9): 1474-1476,1548. doi: 10.16766/j.cnki.issn.1674-4152.002089

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MiR-182 enhances and promotes the proliferation and invasion of melanoma cells

doi: 10.16766/j.cnki.issn.1674-4152.002089

Department of Plastic Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

KJ2020A0576

Received Date: 2021-01-11
Available Online: 2022-02-15

Abstract

Abstract

Objective To investigate the effects of miR-182 on the proliferation and invasion of human melanoma cell line WM-115 and to elucidate its mechanisms of action. Methods Patients with melanoma in our hospital from March 2018 to March 2019 were selected. The expression levels of miR-182 in melanoma tissues and adjacent tissues were detected by real-time quantitative polymerase chain reaction (RT-qPCR). MiR-182 mimics or inhibitor was transfected into WM-115 cells by lipofectamine 2000 package. Cell Counting Kit-8 was applied to detect the viability of WM-115 cells, and transwell chamber assay was used to measure the invasion of WM-115 cells. The expression levels of zinc finger protein 36 like (Zfp36L) mRNA and protein were detected by RT-qPCR and Western blotting, respectively. Results MiR-182 expression in melanoma tissues was significantly higher than that in para-cancer tissues (4.500±1.102 vs. 1.214±0.286, t=7.070, P=0.001). Compared with the control group, miR-182 overexpression significantly increased the proliferation and invasion ability of WM-115 cells (all P < 0.05) and obviously increased the expression levels of Zfp36L mRNA and protein (all P < 0.05), however, miR-182 silencing showed the opposite results. Conclusion The reinforcing effect of miR-182 overexpression on the proliferation and invasion of WM-115 cells may be related to the up-regulation of Zfp36L.
- MiR-182,
- Zinc finger protein homolog 36,
- Melanoma

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MiR-182 enhances and promotes the proliferation and invasion of melanoma cells

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