Volume 19 Issue 7
Jul.  2021
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(7): 1114-1116,1120
ZENG Wei, LIU Yi, LI Wen-ting, LI Yi, ZHU Jin-feng. Bioinformatics analysis of lncRNA DLEU2 transcriptional regulatory factors[J]. Chinese Journal of General Practice, 2021, 19(7): 1114-1116,1120. doi: 10.16766/j.cnki.issn.1674-4152.001998
Citation: ZENG Wei, LIU Yi, LI Wen-ting, LI Yi, ZHU Jin-feng. Bioinformatics analysis of lncRNA DLEU2 transcriptional regulatory factors[J]. Chinese Journal of General Practice, 2021, 19(7): 1114-1116,1120. doi: 10.16766/j.cnki.issn.1674-4152.001998
shu

Bioinformatics analysis of lncRNA DLEU2 transcriptional regulatory factors

doi: 10.16766/j.cnki.issn.1674-4152.001998
  • 1.

    First Department of Lung Cancer Chemotherapy, the Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China

Funds:

 2018D01C255

  • Received Date: 2020-06-09
    Available Online: 2022-02-16
    Abstract
  •   Objective  To investigate the structural characteristics of lncRNA DLEU2 transcription factor and its transcriptional binding sites with lncRNA DLEU2.  Methods  Bioinformatics tools were used to analyse the physical and chemical properties, structure, transmembrane region, signal peptide and subcellular localisation of lncRNA DLEU2 transcriptional regulatory factors and to predict the transcriptional binding sites between transcriptional factors and lncRNA DLEU2.  Results  Isoelectric point analysis showed that the isoelectric points of transcriptional regulatory factors CTCF, MAX, ELF1, YY1 and USF1 were less than 7.0. The isoelectric points of POLR2A, FOXA1, BHLHE40, CEBPB and ATF3 were more than 7.0. Lipolysis index analysis showed that the liposolubility index of the above 10 transcriptional regulatory factors were less than 100, indicating that they were hydrophobic proteins. The results of instability index analysis showed that the instability index of 10 transcriptional regulatory factors in this study were greater than 40, indicating that they were unstable proteins. All the secondary structures of the above transcriptional regulatory factors were composed of α-helix, irregular coiling and extended chain structure, and no transmembrane protein and signal peptide structures were found in the above transcriptional factors. Subcellular localisation analysis showed that YY1 was located in the cytoplasm, and other transcription factors were located in the nucleus. At the same time, the 10 transcriptional regulatory factors and lncRNA DLEU2 transcriptional binding sites were predicted.  Conclusion  The diversity of lncRNA DLEU2 transcriptional regulatory factors may contribute to the further study of the regulation of lncRNA DLEU2 transcriptional expression, and the results will be helpful for the screening of lncRNA DLEU2 related cancer therapeutic drugs.

     

    • FullText(HTML)
  • loading
    • References(22)
  • [1]
    曾薇, 刘翼, 李文婷, 等. 食管鳞癌中转录因子IRF4对miR-133b表达的调控作用研究[J]. 中华全科医学, 2020, 18(11): 1815-1818,1833. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY202011007.htm
    [2]
    SUN Q, HAO Q, PRASANTH K V. Nuclear Long Noncoding RNAs: Key regulators of gene expression[J]. Trends Genet, 2018, 34(2): 142-157. doi: 10.1016/j.tig.2017.11.005
    [3]
    LONG Y, WANG X, YOUMANS T, et al. How do lncRNAs regulate transcription?[J]. Sci Adv, 2017, 3(9): eaao2110. DOI: 10.1126/sciadv.aao2110.
    [4]
    DYKES I M, EMANUELI C. Transcriptional and post-transcriptional gene regulation by long non-coding RNA[J]. Genomics Proteomics Bioinformatics, 2017, 15(3): 177-186. doi: 10.1016/j.gpb.2016.12.005
    [5]
    WANG R J, LI Y, DU P P, et al. Hypomethylation of the lncRNA SOX21-AS1 Has clinical prognostic value in cervical cancer[J]. Life Sci, 2019, 233: 116708. doi: 10.1016/j.lfs.2019.116708
    [6]
    TAN B S, YANG M C, SINGH S, et al. LncRNA NORAD is repressed by the YAP pathway and suppresses lung and breast cancer metastasis by sequestering S100P[J]. Oncogene, 2019, 38(28): 5612-5626. doi: 10.1038/s41388-019-0812-8
    [7]
    QU X, ALSAGER S, ZHUO Y, et al. HOX transcript antisense RNA (HOTAIR) in cancer[J]. Cancer Lett, 2019, 454: 90-97. doi: 10.1016/j.canlet.2019.04.016
    [8]
    THIN K Z, TU J C, RAVEENDRAN S. Long non-coding SNHG1 in cancer[J]. Clin Chim Acta, 2019, 494: 38-47. doi: 10.1016/j.cca.2019.03.002
    [9]
    马杰, 张莉, 向作林. 长链非编码RNA在肝细胞肝癌中的研究进展[J]. 中国临床医学, 2018, 25(1): 117-122. https://www.cnki.com.cn/Article/CJFDTOTAL-LCYX201801030.htm
    [10]
    XIE Z Z, XIAO Z C, SONG Y X, et al. Long non-coding RNA DLEU2 affects proliferation, migration and invasion ability of laryngeal carcinoma cells through triggering miR-16-1 pathway[J]. Eur Rev Med Pharmacol Sci, 2018, 22(7): 1963-1970. http://europepmc.org/abstract/MED/29687850
    [11]
    GIULIETTI M, RIGHETTI A, PRINCIPATO G, et al. LncRNA co-expression network analysis reveals novel biomarkers for pancreatic cancer[J]. Carcinogenesis, 2018, 39(8): 1016-1025. doi: 10.1093/carcin/bgy069
    [12]
    ZHU T G, XIAO X, WEI Q, et al. Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network[J]. Braz J Med Biol Res, 2017, 50(9): e6297. doi: 10.1590/1414-431x20176297
    [13]
    秦延, 陈晓, 赵星, 等. LncRNA DLEU2对胃癌细胞增殖、凋亡和PI3K/Akt信号通路的影响[J]. 临床肿瘤学杂志, 2020, 25(2): 115-120. https://www.cnki.com.cn/Article/CJFDTOTAL-LCZL202002004.htm
    [14]
    KHYZHA N, KHOR M, DISTEFANO P V, et al. Regulation of CCL2 expression in human vascular endothelial cells by a neighboring divergently transcribed long noncoding RNA[J]. Proc Natl Acad Sci U S A, 2019, 116(33): 16410-16419. doi: 10.1073/pnas.1904108116
    [15]
    LIM L J, WONG S Y S, HUANG F, et al. Roles and regulation of long noncoding RNAs in hepatocellular carcinoma[J]. Cancer Res, 2019, 79(20): 5131-5139. doi: 10.1158/0008-5472.CAN-19-0255
    [16]
    URODA T, ANASTASAKOU E, ROSSI A, et al. Conserved Pseudoknots in lncRNA MEG3 are essential for stimulation of the p53 pathway[J]. Mol Cell, 2019, 75(5): 982-995. doi: 10.1016/j.molcel.2019.07.025
    [17]
    赵昕. lncRNA-AK055783通过ERBB2/PI3K/AKT信号通路调控胃癌细胞增殖和侵袭的机制研究[D]. 上海: 第二军医大学, 2017.
    [18]
    XU W, WANG B, CAI Y, et al. DLEU2: A meaningful long noncoding RNA in oncogenesis[J]. Curr Pharm Des, 2020. DOI: 10.2174/1381612826666201026150857.
    [19]
    LU T Y, WANG R, CAI H F, et al. Long Non-Coding RNA DLEU2 promotes the progression of esophageal cancer through miR-30e-5p/E2F7 axis[J]. Biomed Pharmacother, 2020, 123: 109650. doi: 10.1016/j.biopha.2019.109650
    [20]
    WU W M, ZHAO Y H, GAO E J, et al. LncRNA DLEU2 accelerates the tumorigenesis and invasion of non-small cell lung cancer by sponging miR-30a-5p[J]. J Cell Mol Med, 2020, 24(1): 441-450. doi: 10.1111/jcmm.14749
    [21]
    HOU S, QU D J, LI Y, et al. XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence[J]. Nucleic Acids Res, 2019, 47(15): 8239-8254. doi: 10.1093/nar/gkz532
    [22]
    MORRIS J P, YASHINSKIE J J, KOCHE R, et al. α-Ketoglutarate Links p53 to cell fate during tumour suppression[J]. Nature, 2019, 573(7775): 595-599. doi: 10.1038/s41586-019-1577-5
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (385) PDF downloads(9) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return