Volume 19 Issue 6
Jun.  2021
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LIN Chen, CHEN Chuang. Protective effects of Panax notoginseng saponins on P13K/Akt/mTOR signalling pathway and neuronal apoptosis in hippocampus of rats with traumatic brain injury[J]. Chinese Journal of General Practice, 2021, 19(6): 932-935. doi: 10.16766/j.cnki.issn.1674-4152.001953
Citation: LIN Chen, CHEN Chuang. Protective effects of Panax notoginseng saponins on P13K/Akt/mTOR signalling pathway and neuronal apoptosis in hippocampus of rats with traumatic brain injury[J]. Chinese Journal of General Practice, 2021, 19(6): 932-935. doi: 10.16766/j.cnki.issn.1674-4152.001953

Protective effects of Panax notoginseng saponins on P13K/Akt/mTOR signalling pathway and neuronal apoptosis in hippocampus of rats with traumatic brain injury

doi: 10.16766/j.cnki.issn.1674-4152.001953
Funds:

 2020KY005

  • Received Date: 2021-02-25
    Available Online: 2022-02-16
  •   Objective  To investigate the protective effects of Panax notoginseng saponins on the P13K/Akt/mTOR signalling pathway and neuronal apoptosis in the hippocampus of rats with traumatic brain injury (TBI).  Methods  Forty-five SPF SD rats were randomly divided into normal, model and Panax notoginseng saponin groups, and each group contained 15 rats. The Normal group and model group were intraperitoneally injected with the same dose of normal saline, whereas the Panax notoginseng saponin group was intraperitoneally injected with 1% Panax notoginseng saponins (50 mg/kg) once a day for 14 days.  Results  The escape latency of the rats in the Panax notoginseng saponin group [(21.27±3.29)s] was shorter than that in the model group [(28.42±3.87)s], and the number of times they passed the original platform position was (9.73±2.34) times that of the model group [(5.67±1.20) times, all P < 0.05]. The neurobehavioural scores (10.63±2.65) and motor function scores (7.84±2.12) of the rats in the Panax notoginseng saponin group were higher than those in the model group (6.52±1.37 and 4.92±1.35; all P < 0.05). P13K mRNA (0.87±0.18), Akt mRNA (0.92±0.17) and mTOR mRNA (1.02±0.18) levels in the Panax notoginseng saponin group were higher than those in the model group (0.56±0.13, 0.48±0.10 and 0.67±0.21, respectively; all P < 0.05). The Bax protein expression levels (1.57±0.38) in the Panax notoginseng saponins group were lower than those in the model group (3.24±0.54), whereas the Bcl-2 protein expression levels (2.78±0.78) were higher than those in the model group (1.26±0.45; all P < 0.05).  Conclusion  Panax notoginseng saponins have protective effects against neuronal apoptosis in TBI rats, and the mechanism may be related to the upregulation of the P13K/Akt/mTOR signalling pathway and Bcl-2 expression and downregulation of Bax expression.

     

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  • [1]
    SILVERBERG N D, IACCARINO M A, PANENKA W J, et al. Management of concussion and mild traumatic brain injury: A synthesis of practice guidelines[J]. Arch Phys Med Rehabil, 2020, 101(2): 382-393. doi: 10.1016/j.apmr.2019.10.179
    [2]
    宋鸽, 刘晓银, 史新宇, 等. 创伤性脑损伤相关神经炎症的研究进展[J]. 天津医药, 2020, 48(5): 449-454. https://www.cnki.com.cn/Article/CJFDTOTAL-TJYZ202005020.htm
    [3]
    SHARMA S, IFERGAN I, KURZ J E, et al. Intravenous immunomodulatory nanoparticle treatment for traumatic brain injury[J]. Ann Neurol, 2020, 87(3): 442-455. doi: 10.1002/ana.25675
    [4]
    董妍, 聂时南. 创伤性脑损伤预后相关生物标志物的研究进展[J]. 医学研究生学报, 2020, 33(7): 777-780. https://www.cnki.com.cn/Article/CJFDTOTAL-JLYB202007021.htm
    [5]
    马登磊, 张旭, 张丽, 等. 山茱萸环烯醚萜苷对创伤性脑损伤大鼠认知功能及tau蛋白磷酸化的影响[J]. 首都医科大学学报, 2020, 41(3): 397-402. doi: 10.3969/j.issn.1006-7795.2020.03.015
    [6]
    唐三, 丁煌, 杨筱倩, 等. 冰片配伍黄芪甲苷与三七总皂苷对脑缺血再灌注大鼠血脑屏障转运蛋白的影响[J]. 中草药, 2019, 50(18): 4389-4397. doi: 10.7501/j.issn.0253-2670.2019.18.019
    [7]
    欧阳波, 杨筱倩, 丁煌, 等. 冰片配伍黄芪甲苷和三七总皂苷对脑缺血/再灌注损伤后神经血管单元的保护作用[J]. 中国药理学通报, 2020, 36(10): 1470-1476. doi: 10.3969/j.issn.1001-1978.2020.10.025
    [8]
    彭一檬, 余录, 谭华, 等. Micro PET/CT观察三七总皂苷对脑缺血再灌注损伤大鼠模型脑葡萄糖代谢的影响[J]. 中国医学影像技术, 2020, 36(12): 1766-1770. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXX202012003.htm
    [9]
    闫中杰, 赵宗茂, 李凤菊, 等. Toll样受体3预处理增强羊膜间充质干细胞对大鼠创伤性脑损伤的治疗作用[J]. 中华实验外科杂志, 2020, 37(8): 1472-1476. doi: 10.3760/cma.j.cn421213-20200209-01051
    [10]
    KOLOATSOS V E, RAO V. The Behavioral neuroscience of traumatic brain injury[J]. Psychiatr Clin North Am, 2020, 43(2): 305-330. doi: 10.1016/j.psc.2020.02.009
    [11]
    白威, 张欣, 苏鑫洪, 等. 创伤性脑损伤激活miR-124-3p/Notch通路促进大鼠神经干细胞增殖和分化[J]. 细胞与分子免疫学杂志, 2020, 36(1): 49-55. https://www.cnki.com.cn/Article/CJFDTOTAL-XBFM202001009.htm
    [12]
    曾子桓, 魏梁锋, 陈伟强, 等. 鼠神经生长因子对大鼠创伤性脑损伤合并海水淹溺后脑组织β-APP和NF-L表达的影响[J]. 中华航海医学与高气压医学杂志, 2020, 27(2): 147-151. doi: 10.3760/cma.j.cn311847-20190426-00104
    [13]
    童汝有, 董黎明. 三白草木脂素对创伤性脑损伤大鼠神经保护作用及Nrf2-ARE信号通路的影响[J]. 新中医, 2020, 52(6): 1-4. https://www.cnki.com.cn/Article/CJFDTOTAL-REND202006002.htm
    [14]
    杨俊杰, 陈玉军, 于亮, 等. 右美托咪啶对创伤性脑损伤大鼠模型的神经保护作用实验研究[J]. 国际神经病学神经外科学杂志, 2017, 44(5): 523-526. https://www.cnki.com.cn/Article/CJFDTOTAL-GWSK201705016.htm
    [15]
    贾壮壮, 袁庆, 陈红阳, 等. 丹参多酚酸配伍三七总皂苷通过调节PI3K/AKT信号通路对大鼠脑缺血/再灌注损伤神经元凋亡的影响[J]. 中国药理学通报, 2020, 36(9): 1214-1220. doi: 10.3969/j.issn.1001-1978.2020.09.007
    [16]
    丁实, 赵学荣, 李宝群, 等. 三七总皂苷对局部脑缺血大鼠的神经保护作用研究[J]. 中国临床药理学杂志, 2020, 36(9): 1104-1106. https://www.cnki.com.cn/Article/CJFDTOTAL-GLYZ202009016.htm
    [17]
    崔宏, 李静, 王磊, 等. 三七总皂苷对脑组织缺血再灌注损伤的预防作用[J]. 心脑血管病防治, 2020, 20(6): 571-574. doi: 10.3969/j.issn.1009-816x.2020.06.007
    [18]
    王萍, 闫东明, 黄茜, 等. 三七总皂苷治疗缺血性脑卒中的神经保护机制[J]. 中国药理学通报, 2018, 34(12): 1750-1755. doi: 10.3969/j.issn.1001-1978.2018.12.025
    [19]
    党双双, 易甫, 武锋, 等. 芒果苷通过PI3K/Akt/mTOR通路抑制缺氧缺血性脑损伤大鼠神经细胞凋亡及炎症反应[J]. 现代生物医学进展, 2020, 20(10): 1820-1823, 1896. https://www.cnki.com.cn/Article/CJFDTOTAL-SWCX202010005.htm
    [20]
    王亿龙, 刘青峰. 枸杞多糖对大鼠放射性脑损伤海马神经元凋亡的保护作用及其对PI3K/Akt/mTOR信号通路影响[J]. 中国公共卫生, 2019, 35(8): 1043-1045. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGW201908028.htm
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