Volume 19 Issue 6
Jun.  2021
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ZHANG Yi, HU Xiu-juan, DAI Zhi-jun, LIU Chao, HONG Ming-yun. Multiple methods to establish rat endometrial deep injury model[J]. Chinese Journal of General Practice, 2021, 19(6): 913-916,1012. doi: 10.16766/j.cnki.issn.1674-4152.001948
Citation: ZHANG Yi, HU Xiu-juan, DAI Zhi-jun, LIU Chao, HONG Ming-yun. Multiple methods to establish rat endometrial deep injury model[J]. Chinese Journal of General Practice, 2021, 19(6): 913-916,1012. doi: 10.16766/j.cnki.issn.1674-4152.001948

Multiple methods to establish rat endometrial deep injury model

doi: 10.16766/j.cnki.issn.1674-4152.001948
Funds:

 1704a0802171

 J2018Y03

 合卫科教[2019]160号

  • Received Date: 2020-08-05
    Available Online: 2022-02-16
  •   Objective  To establish an animal model of rat endometrial deep injury, and to provide a basis for subsequent treatment of endometrial injury with endometrial stem cells combined with biological scaffolds.  Methods  Fifteen healthy female rats with normal oestrous cycle were randomly divided into the following two groups: 5 rats in the sham operation group and 10 rats in the model group. The female rats in the sham operation group only performed laparotomy without uterus operation. After laparotomy, the left uterus of each rat in the model group received mechanical endometrial scratching, injection of lipopolysaccharide solution and uterine ischemia. The right uterus received surgical incision without any other treatment. After two oestrus cycles, oestrus laparotomy uterine tissues were collected from control group and model group rats. HE and Masson staining and immunohistochemical detection were used for the lining of Collagen Ⅰ; the expression of CK18, Vimentin, PECAM-1 and the damage area ratio of the number of endometrial glands and fibrosis. The expression levels of lining collagen Ⅰ, CK18, Vimentin and PECAM-1 were used for statistical analysis.  Results  Pathological observation showed that the endometrium in the model group was significantly thinner, the number of glands decreased, the structure was disordered, the interstitial fibrous tissue increased and collagen aggregation and fibrosis were obvious. In the immunohistochemical results, the expression level of Collagen Ⅰ in the model group was higher than that in the sham group, whereas the expression levels of CK18, Vimentin and PECAM-1 were lower than that in the sham group, with statistically significant differences (all P < 0.05).  Conclusion  The deep injury model of rat endometrium can be established by mechanical injury, bacterial lipopolysaccharide injection and triple injury after ischemia.

     

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