Volume 19 Issue 5
May  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(5): 731-735
WANG Chao, MEI Zhi-jie, CAO Zhen-xue, LIANG Yu-jie, CHEN Meng-jie, ZHANG Yong-qi, GUO Yuan-yuan, YANG Xiao-huai. Study on the inhibitory effects of miR-140-5p targeting YES1 proto-oncogene[J]. Chinese Journal of General Practice, 2021, 19(5): 731-735. doi: 10.16766/j.cnki.issn.1674-4152.001903
Citation: WANG Chao, MEI Zhi-jie, CAO Zhen-xue, LIANG Yu-jie, CHEN Meng-jie, ZHANG Yong-qi, GUO Yuan-yuan, YANG Xiao-huai. Study on the inhibitory effects of miR-140-5p targeting YES1 proto-oncogene[J]. Chinese Journal of General Practice, 2021, 19(5): 731-735. doi: 10.16766/j.cnki.issn.1674-4152.001903
shu

Study on the inhibitory effects of miR-140-5p targeting YES1 proto-oncogene

doi: 10.16766/j.cnki.issn.1674-4152.001903

  • Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 1808085QH279

  • Received Date: 2020-11-05
    Available Online: 2022-02-16
    Abstract
  •   Objective  To study the targeted regulatory relationship between miR-140-5p and YES1 and elucidate the role of miR-140-5p in regulating the occurrence and development of prostate cancer through YES1. The potential mechanism of action of miR-140-5p in prostate cancer cells was also determined.   Methods  The expression of miR-140-5p in prostate cancer tissues and cell lines was detected via real-time quantitative PCR. The effects of miR-140-5p up-regulation on the proliferation and migration of prostate cancer cells were investigated via cell Counting Kit-8 (CCK-8) assay, cell cloning experiment, and migration and scratch assay. The target gene of miR-140-5p was identified via luciferase reporter assays and Western blotting.   Results  The expression level of miR-140-5p in cancer tissues was significantly down-regulated compared with that in adjacent tissues (P < 0.05). The expression level of miR-140-5p was significantly lower in prostate cancer cell lines than in RWPE-1 cells (P < 0.05). After transfection via CCK-8 assay, the proliferation rate of cells transfected with miR-140-5p significantly decreased, and the number of clones in the hyroid cell clone experiment group was significantly lower than that in the control group (P < 0.05). The healing ability of cell lines transfected with miR-140-5p in the scratch experiments significantly decreased (P < 0.05). Transwell assay revealed that the migration ability of cell lines transfected with miR-140-5p (P < 0.05) decreased, suggesting that overexpression of miR-140-5p can inhibit cell proliferation and migration. Bioinformatics analysis and luciferase report analysis identified YES1 as the potential target gene of miR-140-5p. YES1 overexpression in prostate cancer cells was negatively correlated with miR-140-5p expression, and the difference was statistically significant (P < 0.05).   Conclusion  These experiments demonstrated that miR-140-5p can inhibit the development of prostate cancer by directly targeting YES1, suggesting that miR-140-5p may be a new target for the diagnosis and treatment of prostate cancer.

     

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