Effects of cytoplasmic polyadenylation-element binding protein-4 on the growth of glioma cells

Objective To investigate the expression of Cytoplasmic polyadenylation-element-binding protein 4(CPEB4) in glioma and its effect on glioma cell growth. Methods The expression of CPEB4 protein in glioma tissues, the expression of CPEB4 mRNA in brain glioma tissues and cells, the expression of CPEB4 protein, PIK3CA protein and SMAD3 protein, cell apoptosis, cell cycle, cell proliferation and the invasion ability of cells were detected. Results The positive rate of CPEB4 protein in low-grade brain tissue and high-level brain tissue were higher than that in normal brain tissue(P<0.05). The positive rate of CPEB4 protein in high-level brain tissue was higher than that in low-grade brain tissue(P<0.05). The CPEB4 protein levels in low-level brain tissue and high-level brain tissue were higher than that in normal brain tissue(P<0.05). The level of CPEB4 protein in high-level brain tissue was higher than that in low-grade brain tissue(P<0.05). The expression of CPEB4 protein in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The apoptotic rate in SiRNA-CPEB4 group was significantly higher than that in negative control group and blank control group(P<0.05). The percentage of G₁ phase cells in SiRNA-CPEB4 group was higher than that in negative control group and blank control group(P<0.05). The percentage of S phase cells in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The OD value at 3 days and 7 days in SiRNA-CPEB4 group were lower than that in negative control group and blank control group(P<0.05). The number of invasion cells in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The expression levels of PIK3CA and SMAD3 in SiRNA-CPEB4 group were significantly lower than those in negative control group and blank control group(P<0.05). Conclusion The level of CPEB4 in brain glioma is increased. CPEB4 could affect the apoptosis, cell cycle, proliferation and invasion of glioma cells. The mechanism may be related to PIK3CA and SMAD3.