Abstract: Objective To investigate the expression of microRNA (miR) -187 and its association with clinicopathologic features in the gastric cancer and YNM staging. Methods The miR-187 expression in the 32 cases of gastric cancer and the matched non-tumor adjacent tissue specimens were examined by using stem-loop Real-Time PCR (RT-PCR).The relationship between the expression of miR-187 and its clinicopathologic features of gastric cancer was analyzed by RT-PCR. Results miR-187 was detected in both the tumor tissues and the non-tumor adjacent tissues.The expression level of miR-187 in the tumor tissues was significantly lower than that in the adjacent tissues (P < 0.05).Down-regulated miR-187 expression was associated with the cell differentiation (P < 0.05) and lymph node metastasis TNM staging (P < 0.05) in gastric cancer patients. Conclusions There is a significant difference in the expression level of miR-187 between the tumor tissues and the adjacent tissues (P < 0.05).The miR-187 expression was associated with the cell differentiation and lymph node metastasis TNM staging.The expression of miR-187 might play a key role in the carcinogenesis and progress of gastric cancer.