Abstract: Objective To study the relationship Ezrin protein analysis (Ezrin) and lamin A/C antibody (Lamin A/C) changes in ovarian cancer tissues and clinicopathological features and patient. Methods Select the hospital pathology department collected 30 cases of normal ovarian tissues (normal group),confirmed by pathology after surgical resection specimens of 65 cases of ovarian cancer (ovarian cancer group) and 40 cases of benign ovarian tumor (benign group),using immune expression were detected three groups of specimens Ezrin and Lamin A/C protein and analyze its relationship with clinical and pathological features of patients. Results In normal group,the positive expression of ovarian tissue benign group of Ezrin and Lamin A/C protein,respectively (93.33%,87.50%,93.33%,90.00%) was significantly higher than those in ovarian cancer group (49.23%,52.31%).The difference was statistically significant (P<0.05).Ovarian tissue samples Ⅰ+Ⅱ stage,high+differentiation,Ezrin did not occur in patients with lymph node metastasis and Lamin A/C protein expression was significantly higher than that of Ⅲ+Ⅳ,the positive rate of poorly differentiated ovarian cancer lymph node metastasis,The difference was statistically significant (P<0.05). Conclusion The expression of Ezrin protein and Lamin A/C are closely associated with ovarian cancer metastasis,Ezrin and Lamin A/C protein downregulated in ovarian cancer,and the clinical and pathological characteristics of patients with a certain relationship,It also suggests that Lamin A/C and Ezrin may serve as potential prognostic and prognostic indicators for patients with ovarian cancer.