Abstract: Objective To evaluate the protective effect of dexmedetomidine on perioperative intestinal barrier function in 120 patients with sepsis through comparing the 5 indicators after the operation and 28 day mortality rate. Methods A total of 120 cases of sepsis undergoing surgical treatment in our hospital between March,2014 and September,2015 were enrolled and randomly divided into observation group and control group with 60 cases in each group.In the observation group,dexmedetomidine continuous infusion via an infusion pump was performed 10 minutes before anesthesia (0.6 μg/kg) and after induction of anesthesia[0.5 μg/(kg·h)]until 30 minutes before the end of the surgery;In the control group,normal saline was used instead of dexmedetomidine.The baseline values and postoperative levels (7 days after surgery) of Tumor Necrosis Factor-α(TNF-α),Interleukin 6(IL-6),Plasma levels of Intestinal Fatty Acid Binding Protein (IFABP) Two Amine Oxidase (TAO) and D-Lactic acid (D-Lac) were compared between the two groups.The 28 day mortality rate was also compared between the two groups. Results in the two groups were significantly lower than those before treatment,the difference was statistically significant (P<0.05).The observation group after treatment,the indicators were significantly lower than the control group,the difference was statistically significant (P<0.05).The mortality rate of observation group and control group was 11.67% and 30.00% within 28 days,the difference was statistically significant (P<0.05). Conclusion The levels of TNF-α,IL-6,IFABP,TAO and D-Lac in the observation group were significantly lower than those in the control group at 28 days after the treatment.In the septic group,The postoperative intestinal barrier function and prognosis of a certain protection and improvement.