Abstract: Objective Coronary artery disease (CAD) represents a leading cause of morbidity and mortality worldwide,and genetic factors contribute to the development of this disease.The rate of coronary heart disease showed a rising trend in China.The CYP2C19 gene encodes a member of the cytochrome P450 superfamily of enzymes.It is an important drug metabolizing enzyme.We conducted a case-control study to assess the correlation between gene polymorphism of CYP2C19 and coronary heart disease (CHD) in Yunnan Han population. Methods One hundred and ninety-eight normal persons (control group) and 514 patients of coronary heart disease (CHD group) both written informed consents were recruited from September,2012 to September,2015.Three Polymorphisms rs4986893,rs4244285,rs4917623 in CYP2C19 gene were genotyped using the SEQUENOM Mass-ARRAY system.To investigate the correlation between gene polymorphism of cytochrome P450(CYP)2C19 and coronary heart disease using Logistic regression analysis. Results There were no significant differences in the genotypes and frequencies of alleles of CYP2C19 between the two groups (P>0.05).Logistic regression analysis showed that the rs4986893 A allele was associated with the increased risk of CHD[the dominant model:OR 1.07,95%CI(1.00-1.14),P=0.036]. Conclusion There is correlation between gene polymorphism of CYP2C19 and the risk of coronary heart disease in Yunnan Han Population.Future well designed large studies might be necessary.