Abstract: Objective To investigate the epidemiology of bacterial infections in patients with liver disease and the antimicrobial susceptibility of majority pathogens,and provides theoretical foundation for effective prevention and infection control of the clinical disease. Methods A total of 326 inpatients with liver disease in our hospital were enrolled into this study.The urine,blood and ascites were collected.After isolation and cultivation,the distribution and drug resistance of pathogenics were analyzed.Pathogenics were identified by using VITEK32 automatic bacterial identification system,and the drug susceptibility testing was performed with the use of K-B method.Results referenced to the United States Institute of clinical laboratory standards (CLSI) to determine the standard.Data was analyzed by Excel. Results Total 1 821 strains had been isolated,including 903 Gram-negative strains,accounting for 49.59%,721 Gram-positive strains,accounting for 39.59%,and 151 fungi strains,accounting for 8.29%.The three most common isolates were Escherichia coli (17.63%),Klebsiella pneumonia (14.33%) and coagulase negative Staphylococci (13.23%).The main sites of infection were abdomen (30.5%),respiratory (20.26%) and blood (18.18%).The main causative strain of respiratory infection was Candida albicans.Both Blood infection and peritoneal infection were mainly Escherichia coli.Escherichia coli showed resistant to Piperacillin-tazobactam,but less resistant to Imipenem and Meropenem.The proportion of ESBLS Escherichia coli and Klebsiella pneumonia were 37.21% and 28.62%,respectively.Staphylococcus showed highly resistant to penicillin and erythromycin,but low resistant to vancomycin and teicoplanin. Conclusion The distribution of pathogenic bacteria in patients with severe liver disease is characteristic.The study of distribution and drug resistance of main pathogenics can provide theoretical foundation for the rational use of drugs.