Abstract: Objective To investigate the effects of intensity modulated radiation therapy(IMRT) on the serum levels of TGF-1,IL-8,and T-lymphocyte subsets in patients with nasopharyngeal carcinoma. Methods One hundred and fifteen patients with nasopharyngeal carcinoma were divided into the control group(n=57) and the observation group (n=58) according to the radiation therapy,and the patients were treated with conventional two-dimensional radiotherapy and IMRT.Comparison of two groups of patients with 5 year survival rate and recurrence,adverse reactions,the serum levels of TGF-β1,IL-8,and T lymphocyte subsets were compared before and after treatment. Results ①patients in the observation group showed no regional recurrence survival rate and disease-free survival rate were significantly higher than those in control group(P<0.05),the observation group of patients with local recurrence rate was significantly lower than the control group(P<0.05),observation group of patients with distant metastasis free survival rate was slightly higher than that of control group(P>0.05);②adverse reactions in the control group in the process of radiotherapy in patients with the total incidence rate was 40.35%(23/57),the observation group was 34.48%(20/58),there was no significant difference in the adverse reactions between the two groups(P>0.05);③the serum TGF-beta 1,and IL-8 levels in the two groups of patients after treatment were significantly lower than those before treatment(P<0.05-0.01),and in the observation group,the index level decreased significantly than the control group(P<0.05);④the CD3,CD4 and CD4/CD8 levels in the two groups of patients after treatment were significantly higher than before treatment (P<0.05),CD8 levels after and before treatment were both significantly decreased(P<0.05). Conclusion The effect of IMRT on nasopharyngeal carcinoma is significant,which may be achieved by improving the level of serum TGF-β1,IL-8,and T lymphocyte subsets in patients with nasopharyngeal carcinoma.