Abstract: Objective To investigate the Clinical correlation analysis between JAK2V617 F mutation and myeloproliferative neoplasms(MPN) with BCR/ABL negative gene. Methods Collect 124 patients in my hospital with MPN of BCR/ABL negative,whole blood DNA extracted from peripheral blood or bone marrow,Allele specific PCR technology detect samples whether there is any JAK2V617 F gene locus mutation,combined with clinical data for further analysis. Results Among 124 patients with MPN of BCR/ABL negative,64 cases of male,60 cases of female,the median age of 58(19-83),there has no correlation between JAK2V617 F mutation and gender,and has correlation between JAK2V617 F mutation and age,JAK2V617 F mutation was 70 cases,the positive rate of JAK2V617 F mutation was 56. 45%,the disease of polycythemia vera(PV) patients with positive rate was 62. 07%(18/29),primary thrombocythemia(ET) in patients with positive rate was 53. 57%(45/84 cases),patients with chronic bone marrow fibrosis(IMF) positive rate was 63. 64%(7/11 cases). Conclusion JAK2V617 F mutation can help the diagnosis of MPN with negative BCR/ABL gene. JAK2V617 F mutation can affect hematologic features in some degrees,no correlation between JAK2V617 F mutation and gender,and age have correlation.