Abstract: Objective The morbidity and mortality of hospital-acquired Pseudomonas aeruginosa related with bloodstream infections presented an increased trends recently. This article aimed to analyze clinical distribution and drug resistance of hospital-acquired pseudomonas aeruginosa bacteremia to provide reference for clinical prevention and treatment. Methods The distribution and comorbidity of patients suffered from pseudomonas aeruginosa bacteremia from January,2010 to December,2014 in PLA general hospital were analyzed retrospectively. The susceptibility to antimicrobials of Pseudomonas aeruginosa isolated from blood specimens was tested by Kirby-Bauey methods and the results were performed using WH0 NET 5. 6. At the same time,the differences of drug resistance ratio in recent years were analyzed using SPSS17. 0. Results Total 136 strains of pseudomonas aeruginosa were isolated from blood specimens from January,2010 to December,2014,34 strains(25. 00%) of which distributed in Intensive Care Unit,23 strains(16. 91%) in department of hematology,14 strains(10. 29%) in department of gastroenterology,13 strains(9. 56%) in department of respiratory and so on. The patients with malignant cancers and leukemia were easily affected with bloodstream infections caused by pseudomonas aeruginosa,accounting for 30. 15% and 20. 59%,respectively. Twenty-two strains were multi-drug resistant pseudomonas aeruginosa,the detection rate was 16. 18%. Pseudomonas aeruginosa was seriously resistant to carbopenems,such as imipenem and meropenem,the rates were 33. 09% and 26. 47%,respectively,while Pseudomonas aeruginosa had low resistance rates to amikacin,cefepime,ciprofloxacin,the rates were 13. 24%,16. 18%,18. 38% respectively. The differences of drug resistance ratio of common antibiotics in recent years were not significant(P > 0. 05),but resistance rates of cefoperazone,aztreonam,imipenem,levofloxacin presented increased trends. Conclusion Pseudomonas aeruginosa bacteremia distributes widely in our hospital. The drug resistance of pseudomonas aeruginosa related with bloodstream infections is a bit serious,so drug resistance test should be strengthened and the clinical application of antibiotics should be performed based on the result of drug susceptibility test.