microRNA-34a在肺癌细胞凋亡中的作用

金向宇; 洪卫

doi:10.16766/j.cnki.issn.1674-4152.2016.06.018

microRNA-34a在肺癌细胞凋亡中的作用

doi: 10.16766/j.cnki.issn.1674-4152.2016.06.018

金向宇^,,
洪卫

浙江省肿瘤医院肿瘤内科，浙江杭州 310022

基金项目:

2016年浙江省医药卫生一般研究计划项目(2016-KYB037)

详细信息

通讯作者:
金向宇,E-mail:doctorjin@163.com

中图分类号: R734.2;R329.25
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- 被引次数: 0
出版历程
- 收稿日期: 2015-12-22
- 网络出版日期: 2022-08-06

Role of microRNA-34a in lung cancer cell apoptosis

JIN Xiang-yu^,,
HONG Wei

Department of Oncology,Zhejiang Cancer Hospital,Hangzhou,Zhejiang 310022,China

摘要

摘要: 目的探讨microRNA-34a在肺癌细胞凋亡中的作用。方法将A549肺癌细胞株分为3组：microRNA-34a转染组、对照组和未转染组。分析microRNA-34a在A549肺癌细胞中的表达，microRNA-34a对A549肺癌细胞生长周期、细胞增殖、细胞凋亡的影响，microRNA-34a对A549肺癌细胞Bcl-2、P53和C-MYC蛋白以及mRNA表达的影响。结果 miRNA-34a转染组microRNA-34a的表达明显高于对照组(P<0.05)。miRNA-34a转染组G₀/G₁细胞明显高于对照组和未转染组(P<0.05)。miRNA-34a转染组48 h和96 h的增殖率明显低于对照组和未转染组(P<0.05)。miRNA-34a转染组的细胞凋亡明显高于对照组和未转染组(P<0.05)。miRNA-34a转染组的Bcl-2蛋白和C-MYC蛋白表达水平明显低于对照组(P<0.05)；miRNA-34a转染组的P53蛋白表达水平明显高于对照组(P<0.05)。miRNA-34a转染组的Bcl-2 mRNA和C-MYC mRNA表达水平明显低于对照组Bcl-2 mRNA和C-MYC mRNA表达水平(P<0.05)；miRNA-34a转染组的P53 mRNA表达水平明显高于对照组P53 mRNA表达水平(P<0.05)。结论 microRNA-34a抑制A549肺癌细胞增殖，促进A549肺癌细胞的凋亡，机制可能为下调Bcl-2和C-MYC基因，上调P53基因。
- microRNA-34a /
- 肺癌 /
- 凋亡 /
- 机制
Abstract: Objective To study the role of microRNA-34a in lung cancer cell apoptosis. Methods The A549 lung cancer cell lines were divided into three groups:microRNA-34a transfection group,control group and untransfected group.The expression of microRNA-34a in A549 lung cancer cells,the effect of microRNA-34a on A549 lung cancer cell growth cycle,cell proliferation and apoptosis,and the effect of microRNA-34a on A549 cells Bcl-2,P53 and C-MYC protein expression and mRNA expression were analyzed. Results The expression of miRNA-34a in microRNA-34a transfection group was significantly higher than that of control group(P<0.05).G₀/G₁ cells in miRNA-34a transfection group was significantly higher than that of control group and untransfected group(P<0.05).Forty-eight hours and 96 hours cells proliferation rate in miRNA-34a transfection group was significantly lower than that of control group and untransfected group(P<0.05).Apoptosis in miRNA-34a transfection group was significantly higher than that of control group and untransfected group(P<0.05).Bcl-2 protein and C-MYC protein expression levels in miRNA-34a transfection group were significantly lower than that of control group(P<0.05);P53 protein expression levels in miRNA-34a transfection group was significantly higher than that of control group(P<0.05).Bcl-2 mRNA and C-MYC mRNA expression levels of miRNA-34a transfection group was significantly lower than that of control group(P<0.05);P53 mRNA expression levels of miRNA-34a transfection group was significantly higher than that of control group(P<0.05). Conclusion MicroRNA-34a can inhibit A549 lung cancer cell proliferation and induce A549 lung cancer cells apoptosis.The mechanism may be reducing Bcl-2 and C-MYC gene and increasing P53 gene.
- microRNA-34a /
- Lung cancer /
- Apoptosis /
- Mechanism