Abstract: Objective To learn the drug sensitivity of methicillin-resistant Staphylococcus aureus(MRSA)in our hospital,and provide analysis data for clinical anti-infection treatment and the control of hospital infection. Methods From January,2014 to October,2015,128 cases of sputum,secretion,pus,blood,hydrothorax,ascites,prostatic fluid and urine samples were received,Staphylococcus aureus were detected in all of the specimens.The sensitivity of Staphylococcus aureus to antimicrobial agents was detected,and the sensitivity of MSSA and Methicillin sensitive Staphylococcus aureus(MSSA) to the antimicrobial susceptibility was compared.From 2011 to 2014,Minimum inhibitory concentration(MIC) of 30 strains of MRSA was detected. Results Forty-four strains of MRSA were detected in 128 strains of Staphylococcus aureus,MRSA positive rate was 34.38%;MRSA detection rate of sputum was highest,51.11%;the lowest MRSA detection rate was 20.51% from secretions;there were statistically significant differences in MRSA detection rates of different samples(χ²=12.301,P<0.05).The sensitivity rates of MSSA and MRSA to Quinupristin/dalfopristin,teicoplanin,vancomycin,rifampin,linezolid,tigecycline and nitrofurantoin were 100.00%;The sensitivity rates of MSSA to Oxacillin,Levofloxacin,erythromycin,clindamycin,tetracycline,ciprofloxacin,sulfamethoxazole/trimethoprim and moxifloxacin were significantly higher than that of MRSA,the differences were statistically significant(P<0.01).MIC of Vancomycin and teicoplanin to MRSA showed increasing trend,and MIC of linezolid against MRSA remained unchanged.In 2014,MIC of Vancomycin and teicoplanin to MRSA were significantly higher than in 2011(t=36.242,14.394,P<0.01). Conclusion Staphylococcus aureus isolated from different types of samples have different drug sensitivity and MRSA detection rate,monitoring MIC of glycopeptide antimicrobial agents to MRSA should be pay attention in clinical treatment.