PPARγ激动剂对雄性大鼠脑出血后血肿周围小胶质细胞极化的影响

许莹宁; 穆琼

doi:10.16766/j.cnki.issn.1674-4152.003502

PPARγ激动剂对雄性大鼠脑出血后血肿周围小胶质细胞极化的影响

doi: 10.16766/j.cnki.issn.1674-4152.003502

许莹宁^{1, 2},
穆琼^3, ,

1.
浙江大学附属邵逸夫医院病理科，浙江杭州 310000
2.
贵州医科大学临床医学系，贵州贵阳 550000
3.
贵州医科大学附属医院全科医疗科，贵州贵阳 550000

基金项目:

浙江省医药卫生科技计划项目 2023KY123

贵州医科大学附属医院2020年国家自然科学基金培育项目 gyfynsfc[2020]-2

详细信息

通讯作者:
穆琼，E-mail：591238166@qq.com

中图分类号: R743.34 R-332
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出版历程
- 收稿日期: 2023-09-22

Effect of PPARγ agonists on microglia polarization around hematoma after cerebral hemorrhage in male rats

XU Yingning^{1, 2},
MU Qiong^{3
, ,}

1.
Department of Pathology, Sir Run Run Shaw Hospital Affiliated to Zhejiang University, Hangzhou, Zhejiang 310000, China

摘要

摘要: 目的通过建立大鼠脑出血(intracerebral hemorrhage, ICH)模型，研究PPARγ激动剂(罗格列酮)对雄性SD大鼠脑出血后血肿周围小胶质细胞(M1、M2)极化的影响。方法将48只雄性SD大鼠按照随机数字表法分为正常组(Control)、脑出血模型组(ICH)、氯化钠干预组(NaCl)、罗格列酮干预组(RSG)，每组12只。用大鼠自体血建立ICH模型；各组大鼠分别于6、24、48、72 h时间点进行神经功能评分(Longa评分)，采用免疫组化和光密度法检测血肿周围组织CD16、CD206表达情况。结果各时间点ICH组的Longa评分均高于NaCl组(P<0.05)。各时间点RSG组的Longa评分均低于ICH组(P<0.05)。脑出血造模后6 h RSG组CD16表达(0.226±0.004)较Control组(0.210±0.004)、ICH组(0.221±0.004)和NaCl组(0.220±0.005)的表达均显著升高(P<0.05)。脑出血造模后24 h RSG组CD206表达(0.204±0.004)较Control组(0.184±0.005)、ICH组(0.195±0.005)和NaCl组(0.190±0.006)的表达均显著升高(P<0.05)。结论 PPARγ激动剂罗格列酮能够增强ICH后血肿周围M1、M2型小胶质细胞的表达，特别是促进小胶质细胞向M2型极化。
- 脑出血 /
- 小胶质细胞 /
- PPAR γ激动剂 /
- 罗格列酮 /
- 极化
Abstract: Objective A model of intracerebral hemorrhage (ICH) was established to study the effect of PPARγ agonist (rosiglitazone) on the polarization of microglia (M1 and M2) around the hematoma after intracerebral hemorrhage in male SD rats. Methods A total of 48 male SD rats were divided into four groups according to the random number table method, including the normal group (Control), the intracerebral hemorrhage model group (ICH), the sodium chloride intervention group (NaCl), and the rosiglitazone intervention group (RSG), with 12 rats in each group. The ICH model of male rats was established by autologous blood. The neurological function of all groups of male rats was scored at the time points of 6 h, 24 h, 48 h, and 72 h. The expressions of CD16 and CD206 in the peripheral tissues of hematoma were detected by immunohistochemistry. Results The Longa scores of the ICH group were higher than those of the NaCl group at all time points (P<0.05). The Longa scores of the RSG group were lower than those of the ICH group at all time points (P<0.05). At 6 hours after intracerebral hemorrhage modeling, the CD16 level in the RSG group (0.226±0.004) was higher than that in the control group (0.210±0.004), the ICH group (0.221±0.004), and the NaCl group (0.220±0.005) expression was significantly increased, and the differences were statistically significant(P<0.05). The CD206 in the RSG group (0.204±0.004) was significantly higher than that in the control group (0.184±0.005), the ICH group (0.195±0.005), and the NaCl group (0.190±0.006) at 24 hours after intracerebral hemorrhage modeling(P<0.05). Conclusion PPARγ agonist rosiglitazone can enhance the expression of M1 and M2 microglia around hematoma after ICH, especially promoting the transformation of microglia into M2.
- Intracerebral hemorrhage /
- Microglia /
- PPARγ agonist /
- Rosiglitazone /
- Polarization
利益冲突 无

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图 1 光镜下不同时间点ICH组大鼠脑组织HE染色(×400)

注：A表示ICH组6 h，B表示ICH组24 h，C表示ICH组48 h，D表示ICH组48 h。

Figure 1. Histological images of rat brain tissue in the ICH group at different time points under light microscopy (HE Staining, ×400)

下载: 全尺寸图片幻灯片

表 1 各组大鼠不同时间点神经功能评分(x±s)

Table 1. Neurofunctional scores of each group of rats at different time points (x±s)

组别	例数	6 h	24 h	48 h	72 h
Control组	12	0	0	0	0
NaCl组	12	0	1.0(0.0, 1.5)	1.0(0.0, 1.5)	1.0(0.0, 1.5)
ICH组	12	3.0(1.0, 4.0)^a	2.5(1.5, 3.0)^a	2.5(1.0, 3.0)^a	1.5(1.0, 3.0)^a
RSG组	12	2.5(1.5, 3.0)^ab	1.5(1.0, 3.0)^ab	1.0(0.5, 2.0)^ab	1.0(0.5, 1.5)^ab
注：与NaCl组比较，^aP<0.05；与ICH组比较，^bP<0.05。

下载: 导出CSV

表 2 免疫组化+光密度法检测各组大鼠脑组织不同时间点CD16表达情况(x±s)

Table 2. Immunohistochemistry and densitometry analysis of CD16 expression in rat brain tissue across different time points (x±s)

组别	n	6 h	24 h	48 h	72 h
Control组	12	0.210±0.004^ab	0.208±0.004^ab	0.208±0.004^ab	0.208±0.004
ICH组	12	0.221±0.004^ac	0.215±0.004^c	0.213±0.004^c	0.208±0.005
RSG组	12	0.226±0.004^bc	0.220±0.005^c	0.213±0.004^c	0.211±0.006
NaCl组	12	0.220±0.005^ac	0.218±0.007^c	0.215±0.006^c	0.211±0.005
F值		19.839	7.999	4.123	1.170
P值		0.001	0.001	0.014	0.336
注：与RSG组比较，^aP<0.05；与ICH组比较，^bP<0.05；与Control组比较，^cP<0.05。

下载: 导出CSV

表 3 免疫组化+光密度法检测各组大鼠脑组织不同时间点CD206表达情况(x±s)

Table 3. Detection of CD206 expression in rat brain tissue at various time points using immunohistochemistry and optical density methods (x±s)

组别	n	6 h	24 h	48 h	72 h
Control组	12	0.183±0.006	0.184±0.005^ab	0.184±0.005^a	0.184±0.005^a
ICH组	12	0.183±0.005	0.195±0.005^ac	0.186±0.005^a	0.186±0.005
RSG组	12	0.188±0.005	0.204±0.004^bc	0.194±0.004^bc	0.191±0.006^c
NaCl组	12	0.187±0.010	0.190±0.006^ac	0.191±0.010^c	0.188±0.005
F值		1.392	25.845	4.510	2.813
P值		0.263	0.001	0.010	0.055
注：与RSG组比较，^aP<0.05；与ICH组比较，^bP<0.05；与Control组比较，^cP<0.05。

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