miR-206对腰椎间盘髓核细胞衰老、凋亡的调控机制

朱仲廉; 周平辉; 王照东; 高许斌; 徐陈; 刘亚军; 段克友; 官建中

doi:10.16766/j.cnki.issn.1674-4152.003367

miR-206对腰椎间盘髓核细胞衰老、凋亡的调控机制

doi: 10.16766/j.cnki.issn.1674-4152.003367

朱仲廉^{1, 2},
周平辉^{1, 2},
王照东^{1, 2},
高许斌^{1, 2},
徐陈^{1, 2},
刘亚军^{1, 2},
段克友^{1, 2},
官建中^{1, 2, ,}

1.
蚌埠医科大学第一附属医院骨科，安徽蚌埠 233004
2.
组织移植安徽省重点实验室，安徽蚌埠 233004

基金项目:

安徽省高校自然科学研究项目 2023AH051966

蚌埠医学院自然科学重点项目 2022byzd055

详细信息

通讯作者:
官建中，E-mail: jzguan2002@163.com

中图分类号: R681.53 R-332
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- 文章访问数: 343
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- PDF下载量: 15
- 被引次数: 0
出版历程
- 收稿日期: 2023-08-16
- 网络出版日期: 2024-03-27

Regulation mechanism of miR-206 on senescence and apoptosis of nucleus pulposus cells in lumbar intervertebral disc

ZHU Zhonglian^{1, 2},
ZHOU Pinghui^{1, 2},
WANG Zhaodong^{1, 2},
GAO Xubin^{1, 2},
XU Chen^{1, 2},
LIU Yajun^{1, 2},
DUAN Keyou^{1, 2},
GUAN Jianzhong^{1, 2
, ,}

1.
Department of Orthopedics, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

摘要

摘要: 目的探讨miR-206在腰椎间盘突出症大白兔髓核细胞衰老、凋亡中的调控作用，为腰椎间盘突出症的靶向治疗提供参考。方法选取30只新西兰大白兔作为研究对象，采用自体髓核移植方法构建腰椎间盘突出症大白兔模型，采用随机数字表法分为模型组和对照组，各15只。模型组大白兔注射miR-206模拟剂，对照组注射生理盐水。采用荧光定量聚合酶链反应(qRT-PCR)检测miR-206转录水平，采用细胞染色-β-半乳糖苷酶活性染色方法(SA-β-gal)检测髓核细胞衰老情况，并运用流式细胞仪检测髓核细胞的凋亡情况。结果模型组大白兔髓核细胞miR-206的表达水平(1.65±0.32)明显高于对照组(0.41±0.08)，差异有统计学意义(P＜0.05)；模型组大白兔髓核细胞的阳性细胞数为(14.27±5.36)×10⁵，高于对照组的(5.08±1.13)×10⁵，差异有统计学意义(P＜0.05)；模型组大白兔髓核细胞凋亡率为(8.18±1.94)%，低于对照组的(42.43±6.28)%，差异有统计学意义(P＜0.05)。结论 miR-206在腰椎间盘突出症大白兔髓核细胞中发挥了重要的调控作用，miR-206升高可以减少髓核细胞的衰老现象，降低腰椎间盘突出症大白兔髓核细胞的凋亡率，改善髓核细胞的增殖能力。
- miR-206 /
- 腰椎间盘突出症 /
- 大白兔 /
- 髓核细胞 /
- 衰老 /
- 凋亡
Abstract: Objective To investigate the regulatory effects of miR-206 on senescence and apoptosis of nucleus pulposus cells in white rabbits with lumbar disc herniation, and to provide a reference for targeted therapy of lumbar disc herniation. Methods Thirty cases of New Zealand white rabbits were selected as the study subjects to construct white rabbit model of lumbar disc herniation by autologous nucleus pulposus transplantation. The rabbits were randomly divided into a model group and a control group, with 15 rabbits in each group. The rabbits in the model group were injected with miR-206 simulant, and the rabbits in the control group were injected with normal saline. The transcription level of miR-206 was detected by reverse transcription-polymerase chain reaction (qRT-PCR). Senescence of nucleus pulposus cells was detected by senescence-associated β-galactosidase (SA-β-gal). Nucleus pulposus apoptosis was detected by flow cytometry. Results The expression level of miR-206 in nucleus pulposus cells in the model group (1.65±0.32) was significantly higher than that in the control group (0.41±0.08), the difference was significant (P<0.05). The number of positive cells in the model group[(14.27±5.36) ×10⁵] was higher than that in the control group [(5.08±1.13) ×10⁵], the difference was statistically significant (P<0.05). The apoptosis rate of nucleus pulposus cells in the model group [(8.18±1.94)%] was lower than that in the control group [(42.43±6.28)%], the difference was statistically significant (P<0.05). Conclusion miR-206 plays an important regulatory role in nucleus pulposus cells of white rabbits with lumbar disc herniation. Overexpression of miR-206 can reduce the senescence of nucleus pulposus cells, decrease the apoptosis rate of nucleus pulposus cells in white rabbits with lumbar disc herniation, and improve the proliferation ability of nucleus pulposus cells.
- miR-206 /
- Lumbar disc herniation /
- The big white rabbit /
- Nucleus pulposus cells /
- Aging /
- Apoptosis

HTML全文

表 1 2组腰椎间盘突出症大白兔髓核细胞中miR-206表达水平比较(x±s)

Table 1. Comparison of miR-206 expression levels in nucleus pulposus cells of white rabbits with lumbar disc herniation (x±s)

组别	只数	miR-206
对照组	15	0.41±0.08
模型组	15	1.65±0.32
t值		14.226
P值		<0.001

下载: 导出CSV

表 2 2组腰椎间盘突出症大白兔髓核细胞阳性细胞数比较(x±s，×10⁵)

Table 2. Comparison of the number of positive nucleus pulposus cells in two groups of white rabbits with lumbar disc herniation (x±s, ×10⁵)

组别	只数	髓核细胞阳性细胞数
对照组	15	5.08±1.13
模型组	15	14.27±5.36
t值		28.045
P值		<0.001

下载: 导出CSV

表 3 2组腰椎间盘突出症大白兔髓核细胞凋亡率比较(x±s，%)

Table 3. Comparison of apoptosis rate of nucleus pulposus cells in two groups of white rabbits with lumbar disc herniation (x±s, %)

组别	只数	髓核细胞凋亡率
对照组	15	42.43±6.28
模型组	15	8.18±1.94
t值		38.178
P值		<0.001

下载: 导出CSV

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