Study on the correlations between heat shock protein 70 and severe preeclampsia
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摘要:
目的 观察比较早发型与晚发型重度子痫前期的临床特点，探讨热休克蛋白70(heat shock protein 70，HSP70)与重度子痫前期的相关性。 方法 收集南京市第一医院2013年7月—2020年3月收治的84例早发型重度子痫前期(early-onset severe preeclampsia，EOSP)患者、252例晚发型重度子痫前期(late-onset severe preeclampsia，LOSP)患者的临床资料，比较2组患者的一般情况；应用酶联免疫吸附法检测早发型组，晚发型组及448例正常足月妊娠组血清中HSP70含量，并比较3组HSP70含量的差异。 结果 早发型组与晚发型组的年龄、孕前BMI、发病孕周、分娩孕周、期待治疗天数、收缩压、舒张压、24小时尿蛋白定量(24-hour urinary protein quantity, 24h-Upr)、肌酐(creatinine，Cre)、尿素氮(blood urea nitrogen，BUN)、谷丙转氨酶(alanine transaminase，ALT)、谷草转氨酶(aspartate aminotransferase，AST) 及血小板计数(platelet count，PLT)差异均有统计学意义(均P < 0.05)。早发型组HSP70含量为(101.81±6.44)ng/mL；晚发型组HSP70含量为(86.51±10.70)ng/mL；正常孕妇组中HSP70含量为(51.33±15.48)ng/mL。早发型组HSP70含量高于晚发型组(P < 0.05)，且晚发型组HSP70含量高于正常孕妇组(P < 0.01)。 结论 HSP70在早发型及晚发型重度子痫前期患者血清中的含量明显高于正常孕妇，且早发型组高于晚发型组，表明血清中HSP70可能与重度子痫前期的发病有关。 Abstract:Objective To observe and compare the clinical characteristics of early-onset and late-onset severe preeclampsia (EOSP and LOSP), and to explore the correlation between heat shock protein 70 (HSP70) and severe preeclampsia. Methods The clinical characteristics of 84 EOSP cases and 252 LOSP cases in Nanjing First Hospital from July 2013 to March 2020 were retrospectively analyzed. Enzyme-linked immunosorbent assay was used to detect the serum HSP70 levels of EOSP group, LOSP group and 448 normal full-term pregnancy group. The differences in the results amongst the three groups were compared. Results There were statistical differences in ages, pre-pregnant BMI, onset gestational weeks, delivery gestational weeks, days of expectation treatment, blood pressure (systolic and diastolic blood pressure), 24 h urinary protein quantity (24h-Upr), creatinine (Cre), blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate aminotransferase (AST), and platelet count (PLT) between the EOSP and LOSP groups (all P < 0.05). The values of HSP70 in the EOSP group [(101.81±6.44) ng/mL] was significantly higher than that in the LOSP group [(86.51±10.70) ng/mL, P < 0.05], while the HSP70 levels in the control group [(51.33±15.48) ng/mL] was significantly higher than LOSP group (P < 0.01). Conclusion HSP70 levels in the severe preeclampsia group is significantly higher than that in the normal pregnancy, and it is higher in the EOSP group than that in the LOSP group, indicating that Hsp70 in serum may be related to the pathogenesis of severe preeclampsia. -
Key words:
- Early-onset /
- Late-onset /
- Severe preeclampsia /
- Heat shock protein 70 /
- Serum
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表 1 早发型及晚发型重度子痫前期患者一般情况比较
(x ±s) 组别 例数 年龄
(岁)孕前BMI 发病孕周
(周)分娩孕周
(周)治疗天数
(d)早发型组 84 31.76±4.92 24.96±4.89 30.20±2.95 31.80±3.21 11.17±4.43 晚发型组 252 30.58±5.18 23.65±4.13 36.63±2.51 37.50±2.47 7.32±4.73 t值 2.289 2.644 -25.800 -20.658 7.805 P值 0.023 0.009 ＜0.001 0.002 ＜0.001 
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表 2 早发型及晚发型重度子痫前期患者发病时血压比较
(x ±s，mm Hg) 组别 例数 血压 收缩压 舒张压 早发型组 84 176.10±10.68 115.30±5.00 晚发型组 252 165.31±5.95 111.47±2.15 t值 14.083 11.908 P值 ＜0.001 ＜0.001 注：1 mm Hg=0.133 kPa。
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表 3 早发型及晚发型重度子痫前期患者各项检验指标比较
(x ±s) 组别 例数 24h-Upr(g) Cre(μmol/L) BUN(mmol/L) ALT(U/L) AST(U/L) PLT(×109/L) 早发型组 84 4.89±1.21 99.40±27.53 9.24±1.47 45.67±36.91 54.33±42.35 105.33±17.37 晚发型组 252 3.21±1.34 72.09±19.27 7.51±2.11 34.83±15.76 36.94±16.97 128.61±15.52 t值 13.067 13.504 8.350 5.131 7.351 -13.301 P值 ＜0.001 0.003 0.065 0.216 0.126 ＜0.001
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表 4 3组孕妇血清HSP70含量比较
(x ±s，ng/mL) 组别 例数 HSP70 早发型组 84 101.81±6.43ab 晚发型组 252 86.51±10.70a 正常孕妇组 448 51.33±15.48 F值 860.280 P值 ＜0.001 注：与正常孕妇组比较，aP < 0.01；与晚发型组比较，bP < 0.05。
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