Abstract: Objective To study the effect of health education based on the WeChat public platform "Small Course Assistant" in IVF-ET patients. Methods A total of 200 patients who underwent IVF-ET for the first time at the Reproductive Center of the Second Affiliated Hospital of Wenzhou Medical University from June to December 2018 were selected. According to the time of admission, there were 100 cases in the control group and 100 cases in the research group. The control group was given routine education in the reproductive center. The observation group used a small assistant based on WeChat public platform for education. The negative emotion and disease awareness of the two groups of patients before and after the intervention, and patient compliance were compared. Results After the intervention, the anxiety self-assessment scale(SAS) and depression self-assessment scale(SDS) of the study group were(38.61±5.83) points and(37.78±4.29) points, significantly lower(45.22±6.15) points and(42.16±5.03) points in the control group. The difference was statistically significant(t=-7.802,-6.624, all P<0.05). After the intervention, the disease awareness of the study group was significantly higher than that of the control group. The difference was statistically significant(Z=-5.000, P<0.05). In the study group, the four compliance scores of reasonable diet, medical examination, regular medicine and regular rest and exercise were(5.79±0.39) points,(3.96±0.13) points,(5.97±0.18) points, and(3.90±0.28) points, respectively. Significantly higher than(5.51±0.60) points,(3.90±0.22) points,(5.89±0.28) points,(3.72±0.41) points in the control group. The difference was statistically significant(t=3.912, 2.348, 2.404, 3.626, all P<0.05). Conclusion Health education based on WeChat public platform "Small Course Assistant" can significantly improve the negative emotions of patients with IVF-ET, increase patient awareness and compliance, recommended.