Abstract: Objective To analyze the correlation of vitamin C and E levels in pregnant women with pre-eclampsia by using receiver working curve (ROC curve). Methods A total of 412 pregnant women who were initially examined and delivered in our hospital from January 2016 to January 2017 were analyzed. According to whether or not eclampsia occurred after 20 weeks of gestation, they were divided into preeclampsia group (PE group, 68 cases) and the control group (344 cases). High performance liquid chromatography and enzyme immunoassay for serum vitamin C, vitamin E, pregnancy-related plasma protein A (PAPP-A) in the two groups of pregnant women and pre-pregnancy placental growth factor (PLGF) were tested and compared, and the diagnostic value of pre-eclampsia was predicted by ROC curve analysis of vitamin C and E. Results PAPP-A in the early pregnancy was significantly higher in the two groups than in the second trimester. PLGF was lower than the second trimester (P<0.05), and the PAPP-A in the PE group was significantly higher than that in the control group (t=5.333, 6.225, all P<0.05), PLGF was lower than the control group (t=4.398, 7.771, all P<0.05). The vitamin C and E levels in the second trimester of pregnancy were significantly lower in the two groups than in the first trimester, and the PE group was significantly lower than the control group (all P<0.05); different indicators in the second trimester predict the preeclampsia ROC curve showing that the area under the curve of vitamin E is 0.929 which is significantly higher than vitamin C, PLGF and PAPP-A (AUC=0.836, 0.813, 0.756, all P<0.05), the optimal cut-off point of vitamin E at this time is ≤15.003, the sensitivity is 97.0%, and the specificity is 80.6%. Conclusion The levels of vitamin C and E in pregnant women are significantly decreased in the second trimester, which can effectively predict the occurrence of preeclampsia, and has a higher diagnostic efficiency, while the diagnostic efficacy of vitamin E is higher.