Abstract: Objective To explore whether the blood lipid level is associated with subclinical arteriosclerosis index. Methods A total of 257 subjects from the Health Management Center of the First Affiliated Hospital of USTC Anhui Provincial Hospital were investigated. Their name, age, gender, height, weight, BMI, and blood pressure were collected via questionnaire. The total cholesterol, triglyceride, low density lipoprotein cholesterol, very small low-density lipoprotein cholesterol, high density lipoprotein cholesterol, apolipoprotein A, apolipoprotein B, and lipoprotein a were tested by automatic biochemical analyzer, and the baPWV and ABI were used to evaluate arterial stiffness. The results were calculated by t test and linear regression. Results All the subjects were divided into two groups, including subclinical arteriosclerosis group and control group. Study showed that systolic pressure and diastolic pressure in the subclinical arteriosclerosis group than those in the control group(P<0.05). Both baPWV and ABI showed significant difference between the two groups (P<0.05). To compare serum lipid level, TC, TG, LDL, VLDL and Apo B were statistically significant (P<0.05), while HDL, Apo A and Lpa between two groups indicated no difference (P>0.05). The equation of baPWV is Y₁=12.19X₁+23.07X₂+7.67X₃-156.13(Y₁:baPWV; X₁:age; X₂:TG; X₃:SBP; R=0.740, R₂=0.548, P<0.05). The equation of ABI is Y₂=0.732+0.002X₁+0.002X₂ (Y₂:ABI; X₁ :age; X₂:height; R=0.358, R₂=0.128, F=18.284, P<0.01). Conclusion TC, TG, VLDL and ApoB were influence factors of subclinical arteriosclerosis, while TG is an independent risk factor in the regression equation.