Abstract: Objective To observe and investigate the clinical efficacy of multi-needle sticking in rows acupuncture in protrusion of lumbar intervertebral disc. Methods A total of 90 patients of protrusion of lumbar intervertebral disc who were treated in the Second Affiliated Hospital of Bengbu Medical College from August 2014 to August 2018 were included in the experiment and were randomly and equally divided into 3 groups, with 30 cases in each group. Ninety patients of protrusion of lumbar intervertebral disc were included in the experiment and were randomly and equally divided into 3 groups, with the multi-needle sticking in rows acupuncture as treatment group, and conventional sticking acupuncture and multi-needling acupuncture as controls respectively. The clinical therapeutic effect was assessed by evaluating the clinical symptom, physical sign and symptom pain by VAS (visual analogue scale/score) and JOA (Japanese ortho-paedic association scores) before and after 2 weeks of continuous treatments. The datum were compared among three groups. Results After treatment, the VAS of three groups decreased significantly than that before treatment, with 0.30±0.60 in the multi-needle sticking in rows acupuncture group, 1.43±0.82 in conventional sticking acupuncture group and 1.60±0.77 in multi-needling acupuncture group(all P＜0.01). The VAS of the multi-needle sticking in rows acupuncture group was significantly lower than that in the other groups after treatment(all P＜0.05). In addition, the JOA scores of the multi-needle sticking in rows acupuncture group were 27.73±2.24, which was significantly higher than that in conventional sticking acupuncture group with 23.33±3.37 and multi-needling acupuncture group with 24.10±2.71(all P＜0.01). The JOA scores of all groups after treatments increased significantly than that before treatment(all P＜0.05). With regard to the total therapeutic efficacy, the multi-needle sticking in rows acupuncture group with 90.0% therapeutic efficacy rate was more effective than conventional sticking acupuncture group and multi-needling acupuncture with the therapeutic efficacy rates of 60.0% and 60.0% respectively (all P＜0.05). Conclusion The multi-needle sticking in rows acupuncture with higher therapeutic efficiency in protrusion of lumbar intervertebral disc can effectively relieve the clinical symptom, alleviate uncomfortableness and improve the life quality of patients. Therefore, the popularization of the multi-needle sticking in rows acupuncture will benefit greatly for protrusion of lumbar intervertebral disc patients in clinic.