Abstract: Objective To observe the clinical efficacy of ultramicro needle knife combined with cupping on treatment of patients with hemiplegic shoulder pain, and to provide reliable clinical evidence for the treatment of poststroke hemiplegic shoulder pain. Methods Total 138 patients with hemiplegia and shoulder pain after stroke in our hospital from January 2016 to December 2017 were divided into two groups according to random number method. Treatment group (68 cases) was treated with ultramicro needle knife combined with cupping, and control group (70 cases) was treated with conventional acupuncture. The two groups were treated once every other day, and the therapeutic effect was evaluated after 6 treatments. Before and after treatment, the upper limb Fugl-Meyer, Daily life ability (ADL), pain visual VAS, and life quality of two groups were evaluated. The clinical efficacies of two groups were accounted. The data of two groups were statistically analyzed, to analyze the clinical efficacy of ultramicro needle knife combined with cupping. Results After treatment, the ADL and Fugl-Meyer scores of the two groups were significantly increased (P<0.05), and the ADL and Fugl-Meyer scores of the treatment group were significantly higher than those of the control group (P<0.05). After treatment, the VAS scores of the two groups were significantly lower (P<0.05), and the VAS scores of the treatment group were significantly lower than the control group (P<0.05). After treatment, the life quality scores of two groups were significantly increased (P<0.05), and the life quality scores of the treatment group were significantly higher than those of control group (P<0.05). The clinical effect of the treatment group was better than that of the control group (P<0.05). Conclusion Ultramicro needle knife combined with cupping could effectively treat shoulder pain after stroke, significantly improve the motor function, daily living ability, and life quality.