Abstract: Objective To investigate the influencing factors of intimate relationship with spousal and prenatal depression among patients undergoing assisted reproductive technology, and to improvement of psychological status and health intervention for patients undergoing assisted reproductive technology. Methods From January to December 2017, a total of 426 patients undergoing assisted reproductive technology from five hospitals in Hangzhou were selected, and surveyed by Marital Adjustment Test (MAT) and Edinburgh Postnatal Depression Scale (EPDS). A database of survey questionnaire was established with SPSS 18.0 software was used for date management and analysis. The method of relation analysis was using for analysis of the relation of intimate relationship with spousal and prenatal depression among patients undergoing assisted reproductive technology. Results The scores of MAT of 426 patients undergoing assisted reproductive technology were 104.43±15.85, and a total of 229 patients (53.76%) patients was with better intimate relationship. The scores of EPDS were 8.52±4.18, and the rate of score was 24.41%. The results of Pearson correlation analysis showed that there was a negative correlation between the two (r=-0.395, P=0.008), and the difference was statistically significant. Conclusion The intimate relationship with spousal is negatively correlated with prenatal depression among patients undergoing assisted reproductive technology. The higher the level of intimate relationship, the lower the level of prenatal depression among patients undergoing assisted reproductive technology. The sociology, hospital and family should be paid more attention during the intervention to reduce the level of prenatal depression.