Abstract: Objective To explore the application effect of the physiological driving fidelity human patient simulator in the training of non-technical skills of general practitioner first aid. Methods The 59 general practitioners were selected to carry out first-aid skills training at Xuanwu Hospital of Capital Medical University, who were randomly divided into control group including 29 cases and observation group including 30 cases. The teaching model of traditional theory and simple drill were used in the control group. First aid cases, such as anaphylactic shock, heart failure and cardiac arrest, were designed for general practitioner in the observation group, and the rescue process of these cases were put into the physiological driving fidelity human patient simulator, and the case program was run on the physiological driving fidelity human patient simulator, and the physiological driving fidelity human patient simulator was used to carry out first aid training in the observation group. After training, the anesthesiologist' non-technical skills system (ANTS) were used to evaluate the non-technical skills of 2 groups, the differences of non-technical ability and rescue success rate were compared between the 2 groups. The questionnaire was issued to analyze the evaluation of the effect of the general practitioner on first-aid skills training. Results After training, the score of the task management of the general practitioner in the observation group was 10.29 ±4.53, which was larger than that of the control group 7.65 ±4.67 (P=0.031). The score of decision making ability was 8.42 ±3.82, which was larger than that of the control group 6.12 ±3.73 (P=0.023), and the team cooperation ability score was 13.01 ±4.69, which was higher than that of the control group 9.17 ±4.72 (P=0.003), The score of situational awareness was 8.62 ±3.48, which was higher than that of the control group 6.57 ±3.92 (P=0.038). The questionnaire showed that the scores of the observation group in improving learning interest, improving comprehensive ability and team spirit training were higher than those in the control group (P < 0.05). Conclusion The application of physiological driving fidelity human patient simulator in first-aid skills training could significantly improve the non-technical skills of general practitioners, improve the success rate of rescue, and increase interest in learning and promote team spirit.