MEK/ERK信号通路在上皮性卵巢癌顺铂耐药中的参与作用

张冬雅; 郭红军; 冯巍; 邱海峰

doi:10.16766/j.cnki.issn.1674-4152.000407

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MEK/ERK信号通路在上皮性卵巢癌顺铂耐药中的参与作用

doi: 10.16766/j.cnki.issn.1674-4152.000407

郑州大学第一附属医院妇产科, 河南郑州 450052

基金项目:

2015年度河南省医学科技攻关计划项目(201503009)

详细信息

通讯作者:
张冬雅,E-mail:swzhuhaimian@163.com

中图分类号: R737.31;R730.5
计量
- 文章访问数: 208
- HTML全文浏览量: 29
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2018-01-29
- 网络出版日期: 2022-08-06

Study on the involvement of MEK/ERK signaling pathway in cisplatin-resistance in ovarian cancer

Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China

摘要

摘要: 目的分析MEK/ERK信号通路在上皮性卵巢癌顺铂耐药中的参与作用,为临床治疗提供参考。方法分析郑州大学第一附属医院2015年2月-2017年8月期间诊治的92例卵巢癌顺铂耐药患者(观察组)的临床资料,检测患者MEK/ERK(extracellular regulated protein kinases)信号转导通路相关蛋白的表达。以同期未发生顺铂耐药的卵巢癌患者(对照组)90例为对照。结果卵巢癌顺铂耐药患者血清中MEK/ERK信号通路蛋白Ras、Raf、MEK、ERK1、ERK2及PI3K和AKT蛋白的表达水平明显高于对照组,且差异存在统计学意义(均P<0.05);观察组患者血清中Bcl2 (B-cell lymphoma-2)蛋白的表达水平明显高于对照组,细胞凋亡蛋白Bax(Bcl-2 associated X protein,BAX)和caspase-9(cysteinyl aspartate specific proteinase 9)水平明显低于对照组,且差异存在统计学意义(均P<0.05)。结论卵巢癌顺铂耐药过程可能与体内MEK/ERK信号通路及PI3K和AKT蛋白的异常表达有关。
- MEK/ERK信号通路 /
- 卵巢癌 /
- 顺铂耐药 /
- 细胞凋亡 /
- 表达升高
Abstract: Objective The present research tried to explore the involvement of MEK/ERK signaling pathway in cisplatin-resistance in ovarian cancer. Methods 92 cases of cisplatin-resistance ovarian cancer patients in our hospital from February, 2015 to August, 2017 were enrolled in this part.The expression of MEK/ERK signaling pathway related proteins was detected by ELISA. Ninety cases of ovarian cancer patients without cisplatin-resistance at the same period were taken as control. Results The result revealed that the expression of MEK/ERK signaling pathway related proteins Ras, Raf, MEK and ERK1/2 as well as PI3K/AKT proteins were increased in observation group than control (P<0.05). At the mean time, the Bcl2 were increased (P<0.05) while the expression of Bax and caspase-9 were decreased (P<0.05) in observation group than control with statistical differences. Conclusion MEK/ERK signaling pathway as well as abnormal expression of PI3K and AKT are involved in cisplatin-resistance in ovarian cancer patients.
- MEK/ERK signaling pathway /
- Ovarian cancer /
- Cisplatin-resistance /
- Apoptosis /
- Expression increased