Abstract: Objective To investigate the training needs for general practice trainers. To provide reference and basis for the development of more targeted teacher training programs in the future. Methods The survey was conducted among participants of the training course for general practice trainers hold on August 2016 in Anhui Bozhou according to the cross section research testing method. The contents of questionnaire used for the survey included training needs of general practice relevant knowledge, teaching ability and modes of assessment and training mode and training form. The differences in training needs between general practice trainers from community teaching bases and hospital teaching bases were also analyzed. Results According to the survey, "general practice clinical thinking"(69.5%, 66/95) was the most required relevant knowledge, while "general practice basic concept" was least required 32.6% (31/95). The rate with need of "general practice clinical thinking" in hospital trainers was higher than that in community teaching base trainers (78.8%/58.1%, χ²=4.759, P<0.05). For teaching methods, teaching assessment and evaluation methods, the participants showed more interesting in new-type teaching and evaluation methods, such as PBL (82.1%, 78/95), mini-CEX (52.6%, 50/95) and OSCE (64.2%, 61/95). Teaching experience exchange (85.3%, 81/95), teaching demonstration (73.7%, 70/95), and teaching practice (64.2%, 61/95) were the three most interested modes in current training. Conclusion According to the survey, there are different needs for general practice trainers from hospital and community teaching bases in general practice relevant knowledge, teaching ability and modes of assessment. To meet the different requirements, the focused training course and training method should be adapted targetedly.