Abstract: Objective To investigate the effect of dexmedetomidine on oxidative stress, glycometabolism and postoperative analgesia in patients with laparoscopic radical gastrectomy. Methods According to whether the application of dexmedetomidine, a total of 86 patients with laparoscopic radical gastrectomy were divided into observation group (45 cases) and control group (41 cases) from August, 2014 to October, 2016. The patients of the control group, received conventional general anesthesia, and intravenously pumped with normal saline. The patients of the observation group, received conventional general anesthesia, and intravenously pumped with dexmedetomidine. The changes of oxidative stress (MDA and SOD), glycometabolism (PFK, G-6PD and AR) and postoperative analgesia (VAS scores) were observed and compared before and after operation in 2 groups. Results Compared with before operation, the concentration of MDA increased significantly at 1 d after operation in 2 groups (P<0.05), the concentration of SOD decreased significantly at 1 d after operation in 2 groups(P<0.05), but the changes of observation group was significant smaller (P<0.05). Compared with before operation, the activity of PFK increased significantly at 1 d after operation in 2 groups (P<0.05), the activity of G-6PD and AR decreased significantly at 1 d after operation in 2 groups (P<0.05), but the changes of observation group variation was significant smaller (P<0.05). VAS scores of observation group was significant lower than that in control group at 1 d and 3 d after operation (P<0.05). Conclusion Dexmedetomidine can reduce oxidative stress injury, improve erythrocyte glycometabolism, and enhance the postoperative analgesia in patients with laparoscopic radical gastrectomy.