Effects of different concentrations of isoproterenol on renal pelvic pressure in rabbit perfusion model
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摘要:
目的 采用不同浓度异丙肾上腺素对兔肾脏进行灌注，监测其对兔肾盂压力和生命体征的影响，以探索最佳灌注浓度。 方法 将60只新西兰雄性大白兔采用随机抽签方法分成A、B、C、D四组，每组15只，其中A组、B组、C组为实验组，D组为对照组，实验组分别使用浓度为1.0、0.9、0.8 μg/mL的异丙肾上腺素溶液进行肾盂灌注，D组使用生理盐水进行肾盂灌注。记录灌注0、4、8、16、25 min时各组大白兔的肾盂压、平均动脉压、心率及血药浓度。 结果 3只大白兔因麻醉过深死亡，在A组、B组及D组各1只，得出57只有效实验数据。4组大白兔肾盂灌注后，肾盂压均升高，实验组与对照组比较，差异有统计学意义(P < 0.05);A、B、C三组灌注至4 min后各组肾盂压力开始下降，D组灌注后肾盂压持续升高，在4、8、16、25 min时4组肾盂压力差异有统计学意义(P < 0.05);在16、25 min时，A、B、C三组间差异有统计学意义(P < 0.05)。实验组大白兔肾盂灌注4 min时，平均动脉压、心率、血药浓度数值均高于对照组，差异均有统计学意义(P < 0.05)。 结论 异丙肾上腺素作为灌注液灌注兔肾脏时可降低肾盂压力，浓度为1.0 μg/mL时降低肾盂压力更明显，但影响兔的血压和心率，而在1.0 μg/mL以下均为安全浓度。 Abstract:Objective The rabbit kidney was injected with different concentrations of isoproterenol, and the influence of renal pelvis pressure and vital signs was monitored to explore the optimal perfusion concentration. Methods A total of 60 New Zealand male white rabbits were divided into four groups randomly: A, B, C and D, with 15 rabbits in each group. Among them, A, B and C were the experimental group and D was the control group, 1.0, 0.9, 0.8 μg/mL isoproterenol solution were respectively used for renal pelvis perfusion in the experimental group, while group D received normal saline. Renal pelvis pressure, mean arterial pressure, heart rate and blood drug concentration at the beginning of perfusion, 0, 4, 8, 16, 25 min following perfusion were recorded. Results Three white rabbits died due to excessive anesthesia, one for each group A, B and D, and the experimental data of 57 white rabbits were obtained. After renal pelvis perfusion, the renal pelvis pressure increased in all four groups, and the difference between the experimental groups and the control group was statistically significant (P < 0.05). The renal pelvis pressure of groups A, B and C began to decrease after 4 minutes of perfusion, and the renal pelvis pressure of group D continued to increase after perfusion. The difference in renal pelvic pressure among the four groups at 4, 8, 16, 25 min was statistically significant (P < 0.05). At 16, 25 min, the differences between groups A, B, and C were statistically significant (P < 0.05). After 4 minutes, the mean arterial pressure, heart rate and blood drug concentration of the experimental groups were higher than those of the control group, and the differences were statistically significant (P < 0.05). Conclusion Isoproterenol can reduce the renal pelvis pressure when injected into the rabbit kidney as perfusion solution, more obvious when the concentration was 1.0 μg/mL, but it affects the blood pressure and heart rate of rabbits, whereas the concentration below 1.0 μg/mL is safe. -
Key words:
- Renal pelvis pressure /
- Isoproterenol /
- Heart rate /
- Mean arterial pressure /
- Blood drug concentration
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图 1 实验兔(B组)左侧股动脉置管
Figure 1. Catheterization of the left femoral artery in experimental rabbit (Group B)
图 2 实验兔(C组)左侧输尿管、膀胱、输尿(备注：图 2中文图题中“输尿”疑似不完整，请核对中文图题)
Figure 2. Ureter, bladder, and ureteral orifices of the left side in experimental rabbit (Group C)
图 4 1.0 μg/mL异丙肾上腺素灌注后肾盂压力变化
Figure 4. Changes in renal pelvis pressure following infusion of 1 μg/mL isoproterenol
表 1 4组大白兔不同时间点肾盂内压的比较(x±s, mmHg)
Table 1. Comparison of renal pelvis pressure in four groups of white rabbits at different time points (x±s, mmHg)
组别 只数 0 min 4 min 8 min 16 min 25 min A组 14 2.19±0.65 86.46±4.49a 72.52±7.60ab 44.64±8.71ab 21.72±4.67abcd B组 14 2.65±0.79 91.51±7.15a 84.39±8.50a 58.58±10.51a 42.61±10.64a C组 15 2.09±0.53 88.36±8.64a 75.95±8.10a 65.73±9.50a 50.26±4.29a D组 14 2.64±0.69 96.21±7.61 97.47±7.24 98.49±7.14 99.86±6.44 F值 2.761 4.961 28.072 88.959 318.602 P值 0.053 0.002 < 0.001 < 0.001 < 0.001 注：与灌注0 min比较，aP < 0.01；与同时间点D组比较，bP < 0.01;与同时间点C组比较, cP < 0.01;与同时间点B组比较, dP < 0.01。 
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表 2 4组大白兔不同时间点心率的比较(x±s，次/min)
Table 2. Comparison of heart rate in four groups of white rabbits at different time points (x±s, times/min)
组别 只数 0 min 4 min 8 min 16 min 25 min A组 14 268.1±10.5 275.9±14.8 288.5±15.2a 294.8±10.7a 309.0±15.3a B组 14 269.9±10.4 274.1±9.6 283.0±7.3 290.6±14.0 296.8±9.5 C组 15 269.5±10.2 273.0±21.3 275.4±22.0 279.7±25.4 282.5±16.2 D组 14 271.3±28.2 271.3±22.2 269.2±19.9 273.1±19.7 272.5±19.0 F值 0.161 0.228 3.665 4.063 15.960 P值 0.923 0.876 0.017 0. 007 < 0.001 注：与同时间点D组比较，aP < 0.01。
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表 3 4组大白兔不同时间点平均动脉压的比较(x±s, mmHg)
Table 3. Comparison of mean arterial pressure in four groups of white rabbits at different time points (x±s, mmHg)
组别 只数 0 min 4 min 8 min 16 min 25 min A组 14 59.09±5.83 65.77±5.13 73.31±12.26a 80.66±8.55a 88.94±9.11a B组 14 60.99±3.74 66.41±8.41 70.30±8.04 74.99±8.07 81.56±7.45 C组 15 60.98±6.23 67.69±5.61 70.85±7.40 74.34±8.34 78.57±9.39 D组 14 59.01±3.20 60.85±2.20 60.68±2.88 61.86±2.33 61.27±2.43 F值 1.301 3.842 6.287 16.423 32.768 P值 0.263 0.023 0.001 < 0.001 < 0.001 注：与同时间点D组比较，aP < 0.01。
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表 4 4组大白兔不同时间点血药浓度的比较(x±s, pg/mL)
Table 4. Comparison of blood drug concentration in four groups of white rabbits at different time points (x±s, pg/mL)
组别 只数 0 min 4 min 8 min 16 min 25 min A组 14 3.42±0.51 45.11±8.28a 106.60±10.70a 169.73±12.25a 192.57±25.45a B组 14 3.57±0.41 34.20±8.20 83.06±8.50 101.68±11.26 106.82±18.69 C组 15 3.51±0.43 33.33±6.22 69.97±8.89 78.59±9.61 98.92±9.15 D组 14 3.48±0.46 3.57±0.36 3.53±0.44 3.49±0.42 3.47±0.48 F值 0.261 102.213 409.673 712.923 313.812 P值 0.857 < 0.001 < 0.001 < 0.001 < 0.001 注：与同时间点D组比较，aP < 0.01。
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